Anti-FSH Receptor (extracellular) Antibody

Follicle stimulating hormone receptor, Follitropin receptor, FSH-R, FSHRO
Cat #: AFR-010
Alternative Name Follicle stimulating hormone receptor, Follitropin receptor, FSH-R, FSHRO
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, r
    May also work in: m
      • Peptide (C)SNTGIKHLPAVHK, corresponding to amino acid residues 128-140 of rat FSH Receptor (Accession P20395). Extracellular, N-terminus.
    Accession (Uniprot) Number P20395
    Gene ID 25449
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - 12/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
      • Western blot analysis of rat ovary (lane 1 and 4), rat testis (lane 2 and 5) and human OVCAR3 (lane 3 and 6) lysates:
        1-3. Anti-FSH Receptor (extracellular) Antibody (#AFR-010), (1:200).
        4-6. Anti-FSH Receptor (extracellular) Antibody, preincubated with the control antigen.
      • Expression of FSH receptor in human ovary cell line
        Cell surface detection of FSH receptor in intact living human OVCAR3 cells. A. Extracellular staining of cells using Anti-FSH Receptor (extracellular) Antibody (#AFR-010), (1:25) followed by goat anti-rabbit AlexaFluor-594 secondary antibody. B. Nuclear staining of cells using DAPI as the counterstain. C. Merged images of A and B.
      • FSH (follicle stimulating hormone) is the central hormone of mammalian reproduction, necessary for gonadal development and maturation at puberty and for gamete production during the fertile phase of life1. Together with LH, this gonadotropin is produced and secreted by the pituitary gland as a highly heterogeneous glycoprotein2. The FSH receptor belongs to the family of G-protein coupled receptors, complex transmembrane proteins characterized by seven hydrophobic helices inserted in the plasma membrane and by intracellular and extracellular domains of variable dimensions depending on the type of ligand3. The intracellular portion of FSH receptor is coupled to a Gs protein and, upon receptor activation by the hormonal interaction with the extracellular domain it initiates a cascade of events that ultimately leads to the specific biological effects of the gonadotropin.

        FSH acts by binding to specific receptors, localized exclusively in the gonads. The FSH receptor is expressed by two cell types of the gonad, namely Sertoli cells in the testis, and granulosa cells in the ovarian follicle4.

        Mutations of FSHR may cause primary or secondary amenorrhea, infertility, premature ovarian failure (POF) and ovarian hyperstimulation syndrome (OHSS)5. Mutations of FSHR may also play a role in the development of granulosa cell tumors of ovaries6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 14/04/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat FSH receptor. Anti-FSH Receptor (extracellular) Antibody (#AFR-010) can be used in western blot and is ideally suited to detect the receptor in live cell imaging. It has been designed to recognize FSH Receptor from rat, mouse and human samples.

    For research purposes only, not for human use