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Anti-FSHR (extracellular) Antibody

FSH receptor, Follicle-stimulating hormone receptor, Follitropin receptor, FSHRO

Cat #: AFR-010
Alternative Name FSH receptor, Follicle-stimulating hormone receptor, Follitropin receptor, FSHRO
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
May also work in: m*
Immunogen
  • Peptide (C)SNTGIKHLPAVHK, corresponding to amino acid residues 128-140 of rat FSHR (Accession P20395). Extracellular, N-terminus.
Accession (Uniprot) Number P20395
Gene ID 25449
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - 12/13 amino acid residues identical.
RRID AB_2340935.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, lci, wb
May also work in: ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat ovary (lane 1 and 4), rat testis (lane 2 and 5) and human OVCAR3 (lane 3 and 6) lysates:
    Western blot analysis of rat ovary (lane 1 and 4), rat testis (lane 2 and 5) and human OVCAR3 (lane 3 and 6) lysates:
    1-3. Anti-FSHR (extracellular) Antibody (extracellular) Antibody (#AFR-010), (1:200).
    4-6. Anti-FSHR (extracellular) Antibody, preincubated with FSHR (extracellular) Blocking Peptide (#BLP-FR010).
Live cell imaging / Immunocytochemistry
  • Expression of FSH receptor in human ovary cell line
    Expression of FSH receptor in human ovary cell line
    Cell surface detection of FSH receptor in intact living human OVCAR3 cells. A. Extracellular staining of cells using Anti-FSHR (extracellular) Antibody (#AFR-010), (1:25) followed by goat anti-rabbit AlexaFluor-594 secondary antibody. B. Nuclear staining of cells using DAPI as the counterstain. C. Merged images of A and B.
References
  1. Simoni, M. and Nieschlag, E. (1995) Reprod. Med. Rev. 4, 163.
  2. Moyle, W.R. et al. (1995). De Groot LJ (ed) Endocrinology, 230.
  3. Gudermann, T. et al. (1995) J. Mol. Med. 73, 51.
  4. Simoni, M. et al. (1997) Endocr. Rev. 18, 739.
  5. Lussiana, C. et al. (2008) Obstet. Gynecol. Surv. 63, 785.
  6. Bas, F. et al. (2009) J. Pediatr. Adolesc. Gynecol. 22, 173.
Scientific background

FSH (follicle stimulating hormone) is the central hormone of mammalian reproduction, necessary for gonadal development and maturation at puberty and for gamete production during the fertile phase of life1. Together with LH, this gonadotropin is produced and secreted by the pituitary gland as a highly heterogeneous glycoprotein2. The FSH receptor belongs to the family of G-protein coupled receptors, complex transmembrane proteins characterized by seven hydrophobic helices inserted in the plasma membrane and by intracellular and extracellular domains of variable dimensions depending on the type of ligand3. The intracellular portion of the FSH receptor is coupled to a Gs protein and, upon receptor activation by the hormonal interaction with the extracellular domain it initiates a cascade of events that ultimately leads to the specific biological effects of the gonadotropin.

FSH acts by binding to specific receptors, localized exclusively in the gonads. The FSH receptor is expressed by two cell types of the gonad, namely Sertoli cells in the testis, and granulosa cells in the ovarian follicle4.

Mutations of FSHR may cause primary or secondary amenorrhea, infertility, premature ovarian failure (POF) and ovarian hyperstimulation syndrome (OHSS)5. Mutations of FSHR may also play a role in the development of granulosa cell tumors of ovaries6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat FSH receptor. Anti-FSHR (extracellular) Antibody (#AFR-010) can be used in western blot and live cell imaging applications. It has been designed to recognize the FSH receptor from rat, mouse and human samples.

For research purposes only, not for human use
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