Anti-MICU1 Antibody

Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS
    Cat #: ACC-322
    Alternative Name Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide (C)EFERHDPVDGRISER, corresponding to amino acid residues 313-327 of rat MICU1 (Accession Q6P6Q9). Intracellular.
        Anti-MICU1 Antibody
    Accession (Uniprot) Number Q6P6Q9
    Gene ID 365567
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 14/15 amino acid residues identical.
    RRID AB_2756556.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
      • Anti-MICU1 Antibody
        Western blot analysis of rat skeletal muscle (lanes 1 and 4), mouse kidney (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates:
        1-3. Anti-MICU1 Antibody (#ACC-322), (1:200).
        4-6. Anti-MICU1 Antibody, preincubated with the negative control antigen.
    References
      • Mitochondrial Ca2+ uptake was found to be mediated by the mitochondrial Ca2+ uniporter (MCU). MCU is a ruthenium‐red‐sensitive and highly selective Ca2+ ion channel that uptakes massive amount of Ca2+ across the inner membrane1. MICU1 is a ~54 kDa. protein with an amino‐terminal mitochondrial targeting sequence, a predicted transmembrane helix, and a cytosolic C‐terminus containing two classical EF‐hand Ca2+‐binding domains2.

        Mitochondrial calcium uptake 1 (MICU1) protein was identified as an essential element of mitochondrial Ca2+ uptake. MCU initially takes up Ca2+ very rapidly, but when [Ca2+] inevitably increases inside the matrix, MICU1 binds Ca2+ through its EF‐hand domains, exerting an inhibitory role on MCU‐dependent Ca2+ entry3.

        RNA expression of MICU1 is strongly correlated across a variety of tissues including: stomach, kidney, small and large intestine, cerebral cortex, cerebellum, hypothalamus, spinal cord and skeletal muscles4.

        Loss-of-function mutations in MICU1 causes a brain and muscle disorder linked to primary alterations in mitochondrial Ca2+ signaling5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 18/08/2019

    Anti-MICU1 Antibody (#ACC-322) is a highly specific antibody directed against an epitope of the rat Mitochondrial calcium uptake protein 1 (CBARA1). The antibody can be used in western blot analysis. It has been designed to recognize CBARA1 from rat, mouse, and human samples.

    For research purposes only, not for human use