- Peptide (C)GRNALGRLRLGARR, corresponding to amino acid residues 97-110 of mouse mitochondrial calcium uniporter regulator 1 (Accession Q9CXD6). Intracellular loop, mitochondrial matrix.
- Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat skeletal muscle (lanes 3 and 6) lysates:1-3. Anti-MCUR1 (CCDC90A) Antibody (#ACC-321), (1:200).
4-6. Anti-MCUR1 (CCDC90A) Antibody, preincubated with MCUR1/CCDC90A Blocking Peptide (#BLP-CC321).
Ca2+ flux across the mitochondrial inner membrane regulates bioenergetics, cytoplasmic Ca2+ signals and activation of cell death pathways1. Mitochondrial Ca2+ uptake occurs at regions of close apposition with intracellular Ca2+ release sites driven by the inner membrane voltage generated by oxidative phosphorylation and mediated by a Ca2+ selective ion channel called the uniporter2,3. Mitochondrial calcium uniporter (MCU) was recently identified as an ion-conducting pore4. MCUR1 (mitochondrial calcium uniporter regulator 1), belongs to the CCDC90 family, an integral membrane protein required for MCU-dependent mitochondrial Ca2+ uptake. MCUR1 binds to MCU and regulates ruthenium-red-sensitive MCU-dependent Ca2+ uptake5.
MCUR1 contains two transmembrane helices, where the N- and the short C-termini are exposed to the inter-membrane space5.
MCUR1 is ubiquitously expressed in mammalian tissues including: skeletal muscle, brain, heart, lung, liver, kidney, large intenstine and spleen5.
It was found that cells depleted of MCUR1 have abnormal bioenergetic properties similar to those observed in cells in which Ca2+ uptake is blocked. Thus, MCUR1 is necessary for MCU to efficiently promote mitochondrial Ca2+ uptake and to ensure normal mitochondrial bioenergetics5.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse mitochondrial calcium uniporter regulator 1. Anti-MCUR1 (CCDC90A) Antibody (#ACC-321) can be used in western blot analysis. It has been designed to recognize MCUR1 from mouse and rat samples.