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Anti-MCUR1 (CCDC90A) Antibody

Mitochondrial calcium uniporter regulator 1, MCU regulator 1, Coiled-coil domain-containing protein 90A mitochondrial
Cat #: ACC-321
Alternative Name Mitochondrial calcium uniporter regulator 1, MCU regulator 1, Coiled-coil domain-containing protein 90A mitochondrial
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)GRNALGRLRLGARR, corresponding to amino acid residues 97-110 of mouse mitochondrial calcium uniporter regulator 1 (Accession Q9CXD6). Intracellular loop, mitochondrial matrix.
Accession (Uniprot) Number Q9CXD6
Gene ID 76137
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; human – no apparent homology.
RRID AB_2340895.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat skeletal muscle (lanes 3 and 6) lysates:
    Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat skeletal muscle (lanes 3 and 6) lysates:
    1-3. Anti-MCUR1 (CCDC90A) Antibody (#ACC-321), (1:200).
    4-6. Anti-MCUR1 (CCDC90A) Antibody, preincubated with MCUR1/CCDC90A Blocking Peptide (#BLP-CC321).
References
  1. Orrenius, S. et al. (2003) Nat. Rev. Mol. Cell Biol. 4, 552.
  2. Csordas, G. et al. (2010) Mol. Cell 39, 121.
  3. Santo-Domingo, J. and Demaurex, N. (2010) Biochim. Biophys. Acta 1797, 907.
  4. Baughman, J.M. et al. (2011) Nature 476, 341.
  5. Mallilankaraman, K. et al. (2012) Nat. Cell Biol. 14, 1336.
Scientific background

Ca2+ flux across the mitochondrial inner membrane regulates bioenergetics, cytoplasmic Ca2+ signals and activation of cell death pathways1. Mitochondrial Ca2+ uptake occurs at regions of close apposition with intracellular Ca2+ release sites driven by the inner membrane voltage generated by oxidative phosphorylation and mediated by a Ca2+ selective ion channel called the uniporter2,3. Mitochondrial calcium uniporter (MCU) was recently identified as an ion-conducting pore4. MCUR1 (mitochondrial calcium uniporter regulator 1), belongs to the CCDC90 family, an integral membrane protein required for MCU-dependent mitochondrial Ca2+ uptake. MCUR1 binds to MCU and regulates ruthenium-red-sensitive MCU-dependent Ca2+ uptake5.

MCUR1 contains two transmembrane helices, where the N- and the short C-termini are exposed to the inter-membrane space5.

MCUR1 is ubiquitously expressed in mammalian tissues including: skeletal muscle, brain, heart, lung, liver, kidney, large intenstine and spleen5.

It was found that cells depleted of MCUR1 have abnormal bioenergetic properties similar to those observed in cells in which Ca2+ uptake is blocked. Thus, MCUR1 is necessary for MCU to efficiently promote mitochondrial Ca2+ uptake and to ensure normal mitochondrial bioenergetics5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse mitochondrial calcium uniporter regulator 1. Anti-MCUR1 (CCDC90A) Antibody (#ACC-321) can be used in western blot analysis. It has been designed to recognize MCUR1 from mouse and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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