Anti-MCUR1 (CCDC90A) Antibody

Mitochondrial calcium uniporter regulator 1, MCU regulator 1, Coiled-coil domain-containing protein 90A mitochondrial
    Cat #: ACC-321
    Alternative Name Mitochondrial calcium uniporter regulator 1, MCU regulator 1, Coiled-coil domain-containing protein 90A mitochondrial
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    • Peptide (C)GRNALGRLRLGARR, corresponding to amino acid residues 97-110 of mouse mitochondrial calcium uniporter regulator 1 (Accession Q9CXD6). Intracellular loop, mitochondrial matrix.
    • Anti-MCUR1 (CCDC90A) Antibody
    Accession (Uniprot) Number Q9CXD6
    Gene ID 76137
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - identical; human – no apparent homology.
    RRID AB_2340895.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Anti-MCUR1 (CCDC90A) Antibody
      Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat skeletal muscle (lanes 3 and 6) lysates:
      1-3. Anti-MCUR1 (CCDC90A) Antibody (#ACC-321), (1:200).
      4-6. Anti-MCUR1 (CCDC90A) Antibody, preincubated with the negative control antigen.
    1. Orrenius, S. et al. (2003) Nat. Rev. Mol. Cell Biol. 4, 552.
    2. Csordas, G. et al. (2010) Mol. Cell 39, 121.
    3. Santo-Domingo, J. and Demaurex, N. (2010) Biochim. Biophys. Acta 1797, 907.
    4. Baughman, J.M. et al. (2011) Nature 476, 341.
    5. Mallilankaraman, K. et al. (2012) Nat. Cell Biol. 14, 1336.
    Scientific background

    Ca2+ flux across the mitochondrial inner membrane regulates bioenergetics, cytoplasmic Ca2+ signals and activation of cell death pathways1. Mitochondrial Ca2+ uptake occurs at regions of close apposition with intracellular Ca2+ release sites driven by the inner membrane voltage generated by oxidative phosphorylation and mediated by a Ca2+ selective ion channel called the uniporter2,3. Mitochondrial calcium uniporter (MCU) was recently identified as an ion-conducting pore4. MCUR1 (mitochondrial calcium uniporter regulator 1), belongs to the CCDC90 family, an integral membrane protein required for MCU-dependent mitochondrial Ca2+ uptake. MCUR1 binds to MCU and regulates ruthenium-red-sensitive MCU-dependent Ca2+ uptake5.

    MCUR1 contains two transmembrane helices, where the N- and the short C-termini are exposed to the inter-membrane space5.

    MCUR1 is ubiquitously expressed in mammalian tissues including: skeletal muscle, brain, heart, lung, liver, kidney, large intenstine and spleen5.

    It was found that cells depleted of MCUR1 have abnormal bioenergetic properties similar to those observed in cells in which Ca2+ uptake is blocked. Thus, MCUR1 is necessary for MCU to efficiently promote mitochondrial Ca2+ uptake and to ensure normal mitochondrial bioenergetics5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 20/02/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse mitochondrial calcium uniporter regulator 1. Anti-MCUR1 (CCDC90A) Antibody (#ACC-321) can be used in western blot analysis. It has been designed to recognize MCUR1 from mouse and rat samples.

    For research purposes only, not for human use



    Scientific Background


    Shipping and Ordering information