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Anti-MCT3 Antibody

Monocarboxylate transporter 3, Monocarboxylic acid transporter 3, SLC16A8

Cat #: AMT-013
Alternative Name Monocarboxylate transporter 3, Monocarboxylic acid transporter 3, SLC16A8
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)RSAEGGASDPEDVE, corresponding to amino acid residues 421-434 of rat MCT3 (Accession O70461). Intracellular, C-terminus.
Accession (Uniprot) Number O70461
Gene ID 65200
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical.
RRID AB_2756671.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat whole eye lysate (lanes 1 and 3) and rat hippocampus (lanes 2 and 4):
    Western blot analysis of rat whole eye lysate (lanes 1 and 3) and rat hippocampus (lanes 2 and 4):
    1,2. Anti-MCT3 Antibody (#AMT-013), (1:200).
    3,4. Anti-MCT3 Antibody, preincubated with MCT3 Blocking Peptide (#BLP-MT013).
References
  1. Halestrap, A.P. et al. (1999) Biochem. J. 343, 281.
  2. Gallagher-Colombo, S. et al. (2010) Invest. Ophthalmol. Vis. Sci. 51, 5343.
  3. Daniele, L.L. et al. (2008) Am. J. Physiol. Cell. Physiol. 295, C451.
  4. Philp, N.J. et al. (2001) Am. J. Physiol. Cell. Physiol. 280, C1319.
Scientific background

Proton-linked monocarboxylate transporters (MCTs) are a family of highly homologous trans-membrane symporters, involved in the transportation of monocarboxylic acids across the plasma membrane such as: lactate, pyruvate, branched-chain oxoacids derived from leucine, valine, and isoleucine, ketone bodies, acetoacetate, β-hydroxybutyrate and acetate. Thus, MCTs play an important role in metabolic communication among cells and in mammalian metabolism.

MCT3 is encoded by the Slc16a8 gene and contains at least nine members in humans1. Structurally, MCTs have 12 transmembrane spanning domains with cytoplasmic NH2- and COOH-terminals. The COOH-terminal regions are not well conserved among the MCTs isoforms while the membrane spanning domains share the greatest sequence identity3,4. Findings suggest that the N-terminal domain is more important for energy coupling, membrane insertion and correct structure maintenance, whereas the C-terminal domain is more important for the determination of substrate specificity1.

MCT3 is expressed in the retinal pigment epithelium, preferentially expressed in the basolateral membrane in the adult and in choroid plexus epithelium where it plays a role in regulating pH and lactate concentrations in the outer retina. Reduced levels of MCT3 in response to trauma or disease could contribute to pathologic changes in the retina2,3. MCT3 can form a heteromeric complex with the accessory protein CD147, an immunoglobulin superfamily protein.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-MCT3 Antibody (#AMT-013) is a highly specific antibody directed against an epitope of the rat Monocarboxylate transporter 3. The antibody can be used in western blot analysis. It has been designed to recognize MCT3 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

For research purposes only, not for human use
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