- Peptide (C)DPKHSSVDRSKLH, corresponding to amino acid residues 211-223 of rat MRS2 magnesium transporter (Accession Q9ET09). N-terminus, mitochondrial matrix.
- Western blot analysis of rat testis (lanes 1 and 4) and kidney lysates (lanes 2 and 5) and heart membrane (lanes 3 and 6):1-3. Anti-MRS2 Antibody (#ANT-148), (1:400).
4-6. Anti-MRS2 Antibody, preincubated with MRS2 Blocking Peptide (#BLP-NT148).
Magnesium is one of the most abundant cation in the cell. It is crucial for the proper functioning of various tissues such as the brain, muscle, bone and the nervous system. Currently there are only two types of Mg2+ transport systems which have had their complete structures discovered. MgtE and CorA are the prokaryotic homologues of the SLC41 and MRS2 magnesium transporters.
In eukaryotes, MRS2 protein is expressed in mitochondrial inner membranes. MRS2 is required for normal mitochondrial magnesium homeostasis and for proper myelination in the central nervous system. Notably, the expression of MRS2 serves as a genetic hallmark for embryonic stem cells2.
MRS2 is comprised of two adjacent transmembrane domains. The first domain ends with a gly-met-asn motif and additional secondary structures. A long N-terminal and a short C-terminal sequence are located in the inner side of the mitochondrial membrane leaving the short loop connecting the transmembrane domains protruding to its exterior.
In addition to its Mg2+ permeability, MRS2 transporters somewhat transport Ni2+. However, they are not permeable to Ca2+, Mn2+ and Co2+. MRS2 transporters can be blocked by Cobalt(III)-hexaamine, an analog of the hydrated Mg2+ ion and their activity is regulated by matrix Mg2+ concentrations. Increase in Mg2+ concentration causes a shift of the current-voltage relationship to a more negative potential as expected from the reduction in driving force and significantly reduces the channels’ open-time probability2.
Several SNPs in the MRS2 gene are associated an increased risk of developing type 2 diabetes in women of African American and Hispanic American origin3.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat MRS2 magnesium transporter. Anti-MRS2 Antibody (#ANT-148) can be used in western blot analysis. It has been designed to recognize MRS2 from mouse, rat and human samples.