- Peptide (C)DTVEPDAIKPVGIR, corresponding to amino acid residues 794-807 of mouse CDH2 (Accession P15116). Intracellular.
- Expression of N-Cadherin in mouse heartImmunohistochemical staining of mouse heart free floating frozen sections using Anti-N-Cadherin-ATTO Fluor-594 Antibody (#ANR-082-AR), (1:200). N-Cadherin (red) appears in intercalated discs (arrows). Nuclei are stained with DAPI as the counterstain (blue).
Cadherins are transmembrane proteins responsible for cell adhesion. Cell adhesion is a crucial feature for the sustainability and maintenance of normal tissue function and three dimensional structure. Cadherins share an extracellular domain consisting of multiple repeats of cadherin-specific motif and are calcium dependent homotypic cell-to-cell adhesion molecules. They are localized mostly in specialized sites called adherence junctions1.
N-cadherin consists of an amino-terminal external domain with five tandem repeats, a single transmembrane segment, and a cytoplasmic carboxy-terminal domain of approximately 150 amino acids. The intracellular domain of N-cadherin interacts with a group of proteins called catenins that are essential for cadherin-mediated cell adhesion. It is suggested that N-cadherin proteins align in a form of “zipper” when involved in cell adhesion. Cadherins on one cell surface form a series of rigid dimers that attach to equivalent dimers on the opposing cells and lateral motion of these complexes allows the cell junction site to “zip up”1.
N-cadherin plays a role in tumor metastasis. Unlike E-cadherin that causes tumor metastasis when insufficiently, N-cadherin is involved in tumor metastasis when upregulated. N-cadherin stimulates migration, invasion and metastasis of squamous cell breast cancer and its effects are increased by FGF-2 suggesting that N-cadherin and FGFR-1 synergize and promote aggressive tumor behavior. This synergy also promotes neuronal growth1. Interestingly, low levels of N-cadherin are associated with a higher invasive capacity of astrocytic glioma by increasing tumor (and normal) cell migration speed and creating a less organized pattern of migration3.
Species reactivity key:
Anti-N-Cadherin Antibody (#ANR-082) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize CDH2 from rat, mouse, and human samples.
Anti-N-Cadherin-ATTO Fluor-594 Antibody (#ANR-082-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-N-Cadherin-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.