Anti-Neurokinin Receptor 1 (NK1) (extracellular) Antibody

NK1 Tachykinin receptor, Substance-P receptor Neurokinin1 receptor, Tachykinin receptor 1
    Cat #: ATR-001
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide CMIEWPEHPNRTYEK, corresponding to amino acid residues 180-194 of rat Neurokinin Receptor 1 (NK1) (Accession P14600). 2nd extracellular loop.
    Accession (Uniprot) Number P14600
    Gene ID 24807
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 13/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 mg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, ih, lci, wb
    May also work in: ip
    Western blot
    Western blot analysis of rat ovary (lanes 1 and 5), rat brain (lanes 2 and 6), mouse brain membrane (lanes 3 and 7) and SH-SY5Y (lanes 4 and 8) lysate:
    1-4. Anti-Neurokinin Receptor 1 (NK1) (extracellular) Antibody (#ATR-001), (1:200).
    5-8. Anti-Neurokinin Receptor 1 (NK1) (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Neurokinin Receptor 1 in rat colon
    Immunohistochemical staining of rat colon paraffin-embedded section using Anti-Neurokinin Receptor 1 (NK1) (extracellular) Antibody (#ATR-001), (1:100) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. A. NK1 labeling appears in the tubular glands of the mucosa layer. Note that the smooth muscle and lamina propria do not stain. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
    Indirect flow cytometry
    Indirect flow cytometry analysis of MEG-O1 living cells:
    — Unstained cells.
     Cells + Anti-Anti-Neurokinin Receptor 1 (NK1) (extracellular) Antibody (#ATR-001), (5-10 µg/1x106 cells).
    The control antigen is not suitable for this application.
    Live cell imaging / Immunocytochemistry
    Expression of Neurokinin Receptor 1 (NK1) in rat PC12 cells
    Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-Neurokinin Receptor 1 (NK1) (extracellular) Antibody (#ATR-001), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merge image of A and live view of the cells.
    References
    1. Pennefather, J.N. et al. (2004) Life Sci. 74, 1445.
    2. Hershey, A.D. et al. (1991) J. Biol. Chem. 266, 4366.
    3. Gerard, N.P. et al. (1993) Regul. Pept. 43, 21.
    4. Caberlotto, L. et al. (2003) Eur. J. Neurosci. 17, 1736.
    5. Munoz, M. et al. (2011) Expert Opin. Ther. Targets 15, 889.
    6. Ebner, K. et al. (2009) Curr. Pharm. Des. 15, 1647.
    7. May, A. and Goadsby, P.J. (2001) Expert Opin. Investig. Drugs 10, 673.
    8. Palecek, J. et al. (2003) Neuroscience 116, 565.
    Scientific background

    Substance P (SP), Neurokinin A (NKA) and Neurokinin B (NKB) are all peptides belonging to the Tachykinin protein family. These three peptides which demonstrate a quite heterogeneity in their distribution exert their effect via three receptors: Neurokinin 1-3 receptors, members of the G-protein coupled receptor superfamily. However, Neurokinin 1 Receptor (NK1) preferentially binds Substance P, Neurokinin 2 Receptor (NK2) to NKA and Neurokinin 3 Receptor (NK3) to NKB1.

    Neurokinin receptors are distinguished by their seven transmembrane domains, an extracellular N-terminus and a cytosolic C-terminal. An unusual property of these receptors is the presence of introns as part of their structural organization1-3. Tachykinin receptors undergo alternative splicing. For example, NK1 is detected with different C-terminal lengths. The longer receptor isoform is found in the brain whereas the truncated form is mostly detected in the periphery1,4.

    Due to the broad expression profile of tachykinin peptides, their respective receptors are also expressed in a similar fashion. NK1 is widely expressed in neurons endothelial cells, muscle and immune system cells. NK2 is broadly expressed in the periphery and its expression in the brain is quite restricted. NK3 on the other hand is largely expressed in the central nervous system and is also detected in the uterus, skeletal muscle, lung and liver1.

    Neurokinin receptors have been found in many pathophysiological indications and have therefore become targets for the development of pharmacological compounds. Such indications include cancer, psychological disorders, migraine and various inflammations, just to name a few5-8.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 01/11/2018

    Anti-Neurokinin Receptor 1 (NK1) (extracellular) Antibody (#ATR-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, and live cell imaging applications. It has been designed to recognize NK1 from rat, mouse, and human samples.

    For research purposes only, not for human use
    Citations
    Related Products