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Anti-Neurokinin 2 Receptor (NK2R) Antibody

NK2 receptor, Neurokinin A receptor, Tachykinin receptor 2, TACR2, Substance-K receptor, SKR

Cat #: ATR-002
Alternative Name NK2 receptor, Neurokinin A receptor, Tachykinin receptor 2, TACR2, Substance-K receptor, SKR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide CHTKETLFMTGDMTHSE, corresponding to amino acid residues 351-367 of rat NK2 receptor (Accession P16610). Intracellular, N-terminus.
Accession (Uniprot) Number P16610
Gene ID 25007
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 13/17 amino acid residues identical.
RRID AB_2341078.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat hippocampus (lanes 1 and 3) and rat uterus (lanes 2 and 4):
    Western blot analysis of rat hippocampus (lanes 1 and 3) and rat uterus (lanes 2 and 4):
    1,2. Anti-Neurokinin 2 Receptor (NK2R) Antibody (#ATR-002),  (1:200).
    3,4. Anti-Neurokinin 2 Receptor (NK2R) Antibody, preincubated with Neurokinin 2 Receptor/NK2R Blocking Peptide (#BLP-TR002).
Immunohistochemistry
  • Rat nodose ganglion sections (Yuan, M. et al. (2019) CNS Neurosci. Ther. 25, 123.).

References
  1. Pennefather, J.N. et al. (2004) Life Sci. 74, 1445.
  2. Hershey, A.D. et al. (1991) J. Biol. Chem. 266, 4366.
  3. Gerard, N.P. et al. (1993) Regul. Pept. 43, 21.
  4. Caberlotto, L. et al. (2003) Eur. J. Neurosci. 17, 1736.
  5. Munoz, M. et al. (2011) Expert Opin. Ther. Targets 15, 889.
  6. Ebner, K. et al. (2009) Curr. Pharm. Des. 15, 1647.
  7. May, A. and Goadsby, P.J. (2001) Expert Opin. Investig. Drugs 10, 673.
  8. Palecek, J. et al. (2003) Neuroscience 116, 565.
Scientific background

Substance P (SP), Neurokinin A (NKA) and Neurokinin B (NKB) are all peptides belonging to the Tachykinin protein family. These three peptides, which demonstrate quite a heterogeneity in their distribution, exert their effect via three receptors: Neurokinin 1-3 receptors, members of the G-protein coupled receptor superfamily. However, Neurokinin 1 (NK1) receptor preferentially binds Substance P, Neurokinin 2 (NK2) receptor to NKA, and Neurokinin 3 (NK3) receptor to NKB1.

Neurokinin receptors are distinguished by their seven transmembrane domains, an extracellular N-terminus and a cytosolic C-terminal. An unusual property of these receptors is the presence of introns as part of their structural organization1-3. Tachykinin receptors undergo alternative splicing. For example, NK1 receptor is detected with different C-terminal lengths. The longer receptor isoform is found in the brain whereas the truncated form is mostly detected in the periphery1,4.

Due to the broad expression profile of tachykinin peptides, their respective receptors are also expressed in a similar fashion. NK1 receptor is widely expressed in neurons, endothelial cells, muscle and immune system cells. NK2 receptor is broadly expressed in the periphery and its expression in the brain is quite restricted. NK3 receptor on the other hand is largely expressed in the central nervous system and is also detected in the uterus, skeletal muscle, lung and liver1.

Neurokinin receptors have been found in many pathophysiological indications and have therefore become targets for the development of pharmacological compounds. Such indications include cancer, psychological disorders, migraine and various inflammations, just to name a few5-8.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Neurokinin 2 Receptor (NK2R) Antibody (#ATR-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NK2 receptor from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat nodose ganglion lysate.
    Yuan, M. et al. (2019) CNS Neurosci. Ther. 25, 123.
Immunohistochemistry citations
  1. Rat nodose ganglion sections.
    Yuan, M. et al. (2019) CNS Neurosci. Ther. 25, 123.
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