Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody

NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1
    Cat #: AGP-046
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Guinea pig
    Reactivity: h, m, r
    Immunogen
    Peptide ETEKPRGYQMSTRLK(C), corresponding to amino acid residues 385-399 of rat GluN1 (Accession P35439). Extracellular, N-terminus.
    Accession (Uniprot) Number P35439
    Gene ID 24408
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, mouse - identical
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip, lci
    Western blot
    Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1.2. Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGP-046), (1:200).
    3.4. Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Immuno-colocalization of NMDAR1 and M4 muscarinic receptor in mouse parietal cortex
    Immunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using rabbit Anti-M4 Muscarinic Receptor Antibody (#AMR-004), (1:400) and Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGP-046), (1:1200). A. M4 muscarinic receptor staining (green). B. NMDAR1 staining (red) in same section. C. Merge of the two images reveals several cells expressing both M4 muscarinic receptor and NMDAR1 (arrows). Cell nuclei were stained with DAPI (blue).
    Immuno-colocalization of NMDAR1 and ASCT1 in mouse hippocampus
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGP-046), (1:600) and rabbit Anti-ASCT1 Antibody (#ANT-081), (1:300). A. NMDAR1 (in red) appears in profiles of hippocampal dentate gyrus interneurons (arrows). B. ASCT1 (in green) appears in the extracelullar matrix of the subgranular layer (SGL). C. Merge of the two images demonstrates adjacent localization in several neurons (arrows). Cell nuclei are stained with DAPI (blue). GL = granule layer.
    References
    1. Dingledine, R. et al. (1999) Pharmacol. Rev51, 7.
    2. Mayer, M.L. et al. (2004) Annu. Rev. Physiol66, 161.
    3. Prybylowski, K. et al. (2004) J. Biol. Chem279, 9673.
    4. Mayer, M.L. (2006) Nature 440, 456.
    Scientific background

    The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.

    The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.

    The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.

    Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimer’s disease.

    NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Guinea pig Anti-NMDAR1 (GluN1) (extracellular) AntibodyImmuno-colocalization of GluN1 and Plexin-A1 in rat olfactory bulb.Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGP-046), (1:600) and rabbit Anti-Plexin-A1 (extracellular) Antibody (#APR-081), (1:400). A. NMDAR1 (green) is expressed in the glomeruli (horizontal arrows). B. Plexin A1  staining (red) in the same section, shows expression in the glomeruli (arrow) but also in other layers (vertical arrow) of the olfactory bulb. C. Merge of the two images show cases co-localization in the glomeruli vs. lack of co-localization in deeper layers of the olfactory bulb. Cell nuclei are stained with DAPI (blue).
    Last update: 30/10/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat NMDA Receptor 1. Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGP-046) raised in guinea pig can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting NMDAR1 in living cells. It has been designed to recognize NMDAR1 from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use