Anti-Plexin-A1 (extracellular) Antibody

Semaphorin receptor NOV, PLXN1, PLXNA1
    Cat #: APR-081
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)REVAPITRGQGDQR, corresponding to amino acid residues 623-636 of human Plexin-A1 (Accession Q9UIW2). Extracellular, N-terminus.
    Accession (Uniprot) Number Q9UIW2
    Gene ID 5361
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat - 13/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip, lci
    Western blot
    Western blot analysis of rat (lanes 1 and 3) and  mouse brain synaptosomal fraction  (lanes 2 and 4):
    1, 2. Anti-Plexin-A1 (extracellular) Antibody (#APR-081), (1:400).
    3, 4. Anti-Plexin-A1 (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Immuno-colocalization of GluN1 and Plexin-A1 in rat olfactory bulb
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGP-046), (1:600) and rabbit Anti-Plexin-A1 (extracellular) Antibody (#APR-081), (1:400). A. GluN1 (green) is expressed in the glomeruli (horizontal arrows). B. Plexin A1 staining (red) in the same section, shows expression in the glomeruli (arrow) but also in other layers (vertical arrow) of the olfactory bulb. C. Merge of the two images show cases co-localization in the glomeruli vs. lack of co-localization in deeper layers of the olfactory bulb. Cell nuclei are stained with DAPI (blue).
    References
    1. Turner, L.J. et al. (2004) J. Biol. Chem. 279,199.
    2. Murakami, Y. et al. (2001) Dev. Dyn. 220, 246.
    3. Aci-Seche, S. et al. (2014) PLoS ONE 9, e97779
    4. Ito, T. et al. (2014) Int. J. Mol. Med. 33, 1122.
    Scientific background

    The plexin family are transmembrane receptors, comprises four subfamilies- plexin-A, B, C, and D. In human, three members of the plexin-A subfamily have been isolated- plexin-A1, A2, and A3. Plexin A1 is encoded by the PLXNA1 gene.

    These receptors are characterized by the presence of a conserved intracellular domain of about 600 amino acids known as the sex and plexins domain. This domain is strikingly conserved across the plexin family and consists of two blocks of high sequence conservation separated by a variable linker. The ectodomains of the plexin-A subfamily possess three repeats of the cysteine cluster/Met-Related Sequences.

    Plexins act as signal-transducing components containing transmembrane domain of six Gly residues constituting a GxGGGGG motif.

    Plexins act as functional receptors for members of the semaphorin family. Together they transmit repulsive axon guidance signals for semaphorins inside the axonal growth cone.   The plexins act together with their co-receptors, neuropilins. Plexin-A1, in combination with neuropilins exhibits various functions in axon guidance, organogenesis, angiogenesis, tumorigenesis, and immune regulation1-3.

    Plexin-A1 also plays an important role in the developmental stages of chick heart by working as a receptor for one of the transmembrane semaphorins. In addition Plexin-A1 expressed in dendritic cells is involved in T-dendritic cell interactions in the immune system4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/10/2018

    Anti-Plexin-A1 (extracellular) Antibody (#APR-081) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize Plexin-A1 from mouse, rat and human samples.

    For research purposes only, not for human use