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Anti-NMDAR1 (GluN1) (extracellular) Antibody

NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1
Cat #: AGC-001
Alternative Name NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide ETEKPRGYQMSTRLK(C), corresponding to amino acid residues 385-399 of rat NMDA receptor 1 (Accession P35439). Extracellular, N-terminus.
Accession (Uniprot) Number P35439
Gene ID 24408
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, mouse - identical.
RRID AB_2040023.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001), (1:600).
    2. Anti-NMDAR1 (GluN1) (extracellular) Antibody, preincubated with NMDAR1/GluN1 (extracellular) Blocking Peptide (#BLP-GC001).
Immunohistochemistry
  • Multiplex staining of NMDAR1 and CALHM1 in mouse hippocampal CA1 region
    Multiplex staining of NMDAR1 and CALHM1 in mouse hippocampal CA1 region
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001), (1:200) and Anti-CALHM1-ATTO Fluor-594 Antibody (#ACC-101-AR), (1:60). A. Sections were stained with Anti-NMDAR1 (GluN1) (extracellular) Antibody, followed by goat-anti-rabbit-Cy2 (green). Staining reveals expression in neurons of the pyramidal layer (an arrow points at the layer). B. The same section was incubated with Anti-CALHM1-ATTO Fluor-594 Antibody (red). C. Merge of the two images demonstrates colocalization of NMDAR1 and CALHM1 in pyramidal neurons.
Live cell imaging / Immunocytochemistry
  • Live intact rat hippocampal neurons (1:300). See Mikasova, L. et al. (2012) Brain 135, 1606.
References
  1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7.
  2. Mayer, M.L. and Armstrong, N. (2004) Annu. Rev. Physiol. 66, 161.
  3. Prybylowski, K. and Wenthold, R.J. (2004) J. Biol. Chem. 279, 9673
  4. Mayer, M.L. (2006) Nature 440, 456.
Scientific background

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.

The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.

The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.

Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimer’s disease.

NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-NMDAR1 (GluN1) (extracellular) AntibodyIncreased Synaptic GluN1/mGluR5/Homer1 in Fmr1-/- Mice.Immunocytochemical staining of mouse hippocampal neurons using Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001), (red). Triple immunostaining experiment indicates increased synaptic clustering of GluN1, mGluR5 and Homer1 in Fmr1 knockout mice.Adapted from Aloisi, E. et al. (2017) Nat. Commun. 8, 1103. with permission of SPRINGER NATURE.

Last update: 17/09/2020

Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, live cell imaging, and immunohistochemistry applications. It has been designed to recognize NMDA receptor 1 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Mouse brain lysate (1:500).
    Atkin, G. et al. (2015) J. Neurosci. 35, 6165.
Live cell imaging citations
  1. Rat living and intact hippocampal neurons (1:300).
    Mikasova, L. et al. (2012) Brain 135, 1606.
Immunocytochemistry citations
  1. Mouse hippocampal neurons.
    Aloisi, E. et al. (2017) Nat. Commun. 8, 1103.
  2. Rat hippocampal neurons.
    Oh, J.Y. et al. (2017) Neurosci. Lett. 649, 41.
  3. Human induced pluripotent stem cells (1:300).
    Telezhkin, V. et al. (2016) Am. J. Physiol. 310, C250.
More product citations
  1. Jezequel, J. et al. (2017) Nat. Commun. 8, 1791.
  2. Dupuis, J.P. et al. (2014) EMBO J. 33, 842.
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