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Anti-P2Y12 Receptor (extracellular)-PE Antibody

P2Y purinoceptor 12, P2RY12, P2Y receptor
Cat #: APR-020-PE
Alternative Name P2Y purinoceptor 12, P2RY12, P2Y receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2Y12 receptor (Accession Q9H244). 3rd extracellular loop.
Accession (Uniprot) Number Q9H244
Gene ID 64805
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse – 12/13 amino acid residues identical.
RRID AB_2876814
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label R-Phycoerythrin (R-PE).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-020), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
  • Cell surface detection of P2Y12 in live intact mouse BV-2 microglia cells:
    Cell surface detection of P2Y12 in live intact mouse BV-2 microglia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-PE.
    ___ Cells + Anti-P2Y12 Receptor (extracellular)-PE Antibody (#APR-020-PE), (2.5 µg).
  • Cell surface detection of in live intact human MEG-01 megakaryoblastic leukemia cells:
    Cell surface detection of in live intact human MEG-01 megakaryoblastic leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-PE.
    ___ Cells + Anti-P2Y12 Receptor (extracellular)-PE Antibody (#APR-020-PE), (5 µg).
References
  1. Cattaneo, M. (2015) J. Thromb. Haemost. 13, S10.
Scientific background

The P2Y receptors are a widely expressed group of G-protein coupled receptors (GPCRs). P2Y receptors are activated by nucleotides which are extracellular signaling molecules that are released from damaged cells or secreted via non-lytic mechanisms during inflammation, ischemia or hypoxia.

P2Y12 receptor, like all P2Y receptors, is a 7-membrane spanning protein coupled to a G protein. Most of the seven transmembrane helices of P2YR12 are not perpendicular to the plane of the membrane but are tilted or kinked. The carboxy-terminal helix VIII is parallel to the membrane lipid bilayer. The receptor contains 342 amino acid residues and two potential N-linked glycosylation sites at its extra-cellular amino terminus, which may modulate its activity.

ADP and some of its analogues stimulate P2YR12-mediated inhibition of adenylyl cyclase through activation of the Gαi2 G protein subtype, although effective coupling may also occur to Gαi1 G and Gαi3 G.

P2YR12 is an important cofactor of platelet aggregation and secretion induced by several agonists including collagen, TXA2, thrombin and specific antibodies cross-linking FcγRIIa receptors. It also plays an important role in shear-induced platelet aggregation and in platelet thrombus formation and stabilization on collagen-coated surfaces or ruptured atherosclerotic plaques under flow conditions.

Inherited abnormalities of P2YR12 include an autosomal recessive disorder causing severe receptor deficiency. Due to this abnormality, ADP, even at very high concentrations, does not induce full and irreversible platelet aggregation, nor does it inhibit the stimulation of platelet adenylyl cyclase causing excessive bleeding and additional coagulative dysfunction.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 20/10/2020

Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) is a highly specific antibody directed against an extracellular epitope of the human P2RY12. The antibody can be used in western blot, live cell imaging and indirect flow cytometry applications. It has been designed to recognize P2RY12 from human, rat and mouse samples.

Anti-P2Y12 Receptor (extracellular)-PE Antibody (#APR-020-PE) is directly conjugated to Phycoerythrin (PE). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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