Anti-Parathyroid Hormone 1 Receptor (extracellular) Antibody

PTH/PTHrP type I receptor, PTH1 receptor, PTHR, PTHR1
    Cat #: APR-051
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)DAVLYSGFTLDEAER, corresponding to amino acid residues 241-255 of rat Parathyroid Hormone 1 Receptor (Accession P25961 ). 1st extracellular loop.
    Accession (Uniprot) Number P25961
    Gene ID 56813
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 14/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of HepG2 (lanes 1 and 4), mouse liver (lanes 2 and 5) and rat liver (lanes 3 and 6) lysates:
    1-3. Anti-Parathyroid Hormone 1 Receptor (extracellular) Antibody (#APR-051), (1:200).
    4-6. Anti-Parathyroid Hormone 1 Receptor (extracellular) Antibody preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Parathyroid hormone receptor 1 in rat brain
    Immunohistochemical staining of rat dorsal Raphe nucleus using Anti-Parathyroid Hormone Receptor 1 (extracellular) Antibody (#APR-051). A. PTH1R (red) staining is detected in Raphe neurons (arrows). B. Nucleus staining using DAPI as the counterstain (blue). C. Merged images of A and B.
    Immuno-colocalization of Parathyroid Hormone 1 Receptor and Orexin Receptor 1 in rat ventromedial hypothalamus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Orexin Receptor 1-ATTO-488 Antibody (#AOR-001-AG), (1:60) and Anti-Parathyroid Hormone 1 Receptor (extracellular) Antibody (#APR-051), (1:60). A. Sections were incubated Anti-Parathyroid Hormone 1 Receptor (extracellular) antibody, followed by donkey-anti-rabbit-Cy3 (red). B. The same sections were incubated with Anti-Orexin Receptor 1-ATTO-488 antibody, (green). C. Merge of A and B shows staining of Orexin Receptor 1 in nerve fibers (vertical arrows) while Parathyroid Hormone 1 Receptor is apparent on cell bodies (horizontal arrows). Cell nuclei are stained with DAPI (blue).
    Live cell imaging / Immunocytochemistry
    Expression of Parathyroid Hormone 1 Receptor in rat U-87 MG cells
    Immunocytochemical staining of intact living rat U-87 MG cells. A. Extracellular staining of cells using Anti Parathyroid Hormone 1 Receptor (extracellular) Antibody (#APR-051), (1:50), (green). B. Merge of A with live view of the cells.
    References
    1. Potts, J.T. and Gardella, T.J. (2007) Ann. N.Y. Acad. Sci. 1117, 196.
    2. Vilardaga, J.P. et al. (2011) Cell. Mol. Life Sci. 68, 1.
    3. Potts, J.T. (2005) J. Endocrinol. 187, 311.
    4. Chung, U.I. et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 13030.
    5. Vortkamp, A. et al. (1996) Science 273, 613.
    6. Mahon, M.J. et al. (2006) Mol. Endocrinol. 20, 136.
    7. Singh, A.T. et al. (2005) Endocrinology 146, 2171.
    8. Schipani, E. et al. (1995) Science 268, 98.
    9. Bastepe, M. et al. (2004) J. Clin. Endocrinol. Metab. 89, 3595.
    10. Duchatelet, S. et al. (2005) Hum. Mol. Genet. 14, 1.
    Scientific background

    The parathyroid hormone receptor type 1 (PTHR1) is bound and activated by two peptide hormones: the parathyroid hormone (PTH) secreted by the parathyroid gland and by parathyroid hormone related peptide (PTHrP) which is produced locally in many tissues. Although these two peptides bind the same receptor, they exert different actions. PTH regulates the concentrations of Ca2+, phosphate ions and vitamin D in the blood, stimulates bone formation/resorption and is distributed to the kidneys where it regulates Ca2+ and phosphate transport, and is also detected in the liver1-3. PTHrP, on the other hand, plays a critical role early in development of bone, heart, mammary glands and other tissues, and is detected in the circulation only during lactation and secreted by some tumors1,2,4,5. PTHR1 is obviously localized to all areas and tissues where PTH and PTHrP are known to exert their effects (i.e. bone, kidney, blood, heart, etc.).

    PTHR1 belongs to the superfamily of G-protein coupled receptors. Like all members, PTHR1 has seven transmembrane spanning domains, an extracellular N-terminus and an intracellular C-terminal tail. Upon activation, the receptor couples to either Gs and Gq thereby activating adenylate cyclase and phospholipase C (PLC) respectively. In osteosarcoma cells, PTHR1 can also couple Gα2,6,7.

    PTH is being used today to treat osteoporosis, as a factor promoting bone formation. Mutations in PTHR1 are associated with Jansen’s disease (hypercalcemia and dwarfism) and Eiken syndrome (cartilage tumors of bone)2,8-10.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 01/11/2018

    Anti-Parathyroid Hormone 1 Receptor (extracellular) Antibody (#APR-051) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize PTHR1 from human, mouse, and rat samples.

    For research purposes only, not for human use