Free shipping starts now, no minimum, no coupons required!

Anti-SLC22A2 (OCT2) Antibody

Organic cation transporter 2, Solute Carrier Family 22 Member 2

Cat #: ACT-020
Alternative Name Organic cation transporter 2, Solute Carrier Family 22 Member 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (CN)LTADEDTGMKLNPS, corresponding to amino acid residues 321 - 334 of mouse SLC22A2 (Accession O70577). Intracellular, 3rd loop.
Accession (Uniprot) Number O70577
Gene ID 20518
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry
Homology Mouse – 14 out of 15 amino acid identical; Rat- 12 out of 15 amino acid residues identical. It won't recognize human SLC22A2.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
SLC22A2 (OCT2) Blocking Peptide (#BLP-CT020)
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse kidney lysates (lanes 1 and 3) and rat kidney lysates (lanes 2 and 4):
    Western blot analysis of mouse kidney lysates (lanes 1 and 3) and rat kidney lysates (lanes 2 and 4):
    1-2. Anti-SLC22A2 (OCT2) Antibody (#ACT-020), (1:200).
    3-4. Anti-SLC22A2 (OCT2) Antibody, preincubated with SLC22A2 (OCT2) Blocking Peptide (BLP-CT020).
  • Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1-2. Anti-SLC22A2 (OCT2) Antibody (#ACT-020), (1:200).
    3-4. Anti-SLC22A2 (OCT2) Antibody, preincubated with SLC22A2 (OCT2) Blocking Peptide (BLP-CT020).
Immunohistochemistry
  • Expression of OCT2 in rat medial septum nucleus.
    Expression of OCT2 in rat medial septum nucleus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-SLC22A2 (OCT2) Antibody (#ACT-020), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. OCT2 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with SLC22A2 (OCT2) Blocking Peptide (BLP-CT020), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Nigam, S.K. (2018) Annu Rev Pharmacol Toxicol. 58: 663.
  2. Hall, M.D., et al. (2008) Annu Rev Pharmacol Toxicol, 48: 495.
  3. Koepsell, H. and H. Endou. (2004) Pflugers Arch, 447: 666.
  4. Koepsell, H., et al. (2003). Rev Physiol Biochem Pharmacol, 150: 36.
  5. Yonezawa, A., et al. (2005) Biochem Pharmacol, 70: 1823.
  6. Koepsell, H. et.al. (2020) Pharmacol. Rev. 72, 253.
Scientific background

Organic cation transporters are members of the SLC22A superfamily. These transporters are polyspecific and are involved in the absorption and excretion of various endogenous and exogenous compounds1. The family consists of 18 genes and includes the organic cation transporters (OCTs), organic cation/carnitine transporters (OCTNs), and organic anion transporters (OATs)1-3.

Most family members share a common membrane topology consisting of a predicted 12 α-helical transmembrane domains and two hydrophilic loops2,4. Although they share a common structure, the transporters in the SLC22A family show differential tissue distribution. The genes encoding the three organic cation transporters (SLC22A1-3) are all located in a cluster on chromosome 62-4.

Organic cation transporter 2 (OCT2) was first identified in the rat in 1996. It is primarily located on the basolateral surface of renal proximal tubules, particularly on the S2 and S3 segments and also expressed in brain.

OCT2 mediates the uptake from circulation of several endogenous compounds like creatinine, choline and norepinephrine and also exogenous ones, that include commonly prescribed drugs like the antineoplastic drug cisplatin and the antidiabetic Metformin 5,6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Anti-SLC22A2 (OCT2) Antibody (#ACT-020) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC22A2 from rat and mouse samples. The antibody won’t recognize human OCT2.

For research purposes only, not for human use
Shipping and Ordering information