Free shipping starts now, no minimum, no coupons required!

Anti-ENT3 (SLC29A3) Antibody

Equilibrative nucleoside transporter 3

Cat #: ANT-053
Alternative Name Equilibrative nucleoside transporter 3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)DYPAPGLQRPEDR, corresponding to amino acid residues 37-49 of rat ENT3 (Accession Q80WK7). Intracellular, N-terminus.
Accession (Uniprot) Number Q80WK7
Gene ID 353307
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 10/13 amino acid residues identical.
RRID AB_2756717.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat liver lysate (lanes 1 and 4), mouse liver lysate (lanes 2 and 5) and rat kidney membranes (lanes 3 and 6):
    Western blot analysis of rat liver lysate (lanes 1 and 4), mouse liver lysate (lanes 2 and 5) and rat kidney membranes (lanes 3 and 6):
    1-3. Anti-ENT3 (SLC29A3) Antibody (#ANT-053), (1:200).
    4-6. Anti-ENT3 (SLC29A3) Antibody, preincubated with ENT3/SLC29A3 Blocking Peptide (#BLP-NT053).
References
  1. Liu, B. et al. (2015) Biochim. Biophys. Acta 1852, 2086.
  2. Rahman, M.F. et al. (2017) J. Biol. Chem. 292, 14775.
Scientific background

Equilibrative nucleoside transporter 3 (ENT3) is responsible for transporting nucleotide synthesis precursors, such as hydrophilic nucleosides and nucleobases within cells1. ENT3 structure includes 11 transmembrane domains and a 51-residue hydrophilic N-terminal region containing two dileucine motifs that are characteristic of endosomal/lysosomal targeting sequences1,2.

ENT3 is encoded by the SLC29A3 gene and is expressed in several mouse and human tissues such as placenta, heart, neurons, and astrocytes. In addition, human ENT3 is predominantly expressed in mitochondria in human hepatocytes. Human ENTs are high-capacity transporters, bidirectional, Na+-independent with a wide substrate selectivity1,2

The human ENT family consists of four members: ENT1- ENT4. Nucleoside transport function of ENT3 is maximal at pH 5.5 suggesting that the protein is localized to acidic intracellular compartments; probably lysosomes1,2.

Studies show that mutations in human ENT3 can cause various clinical disorders including H syndrome, pigmented hypertrichosis with insulin-dependent diabetes mellitus syndrome, lysosomal storage-like disorders and mitochondrial disorders1,2

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-ENT3 (SLC29A3) Antibody (#ANT-053) is a highly specific antibody directed against an epitope of the rat equilibrative nucleoside transporter 3. The antibody can be used in western blot analysis. It has been designed to recognize ENT3 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Shipping and Ordering information

Resources