Anti-Somatostatin Receptor Type 2 (extracellular) Antibody

SSTR2, SRIF1A
    Cat #: ASR-006
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)GSNQTEPYYDMTSN, corresponding to amino acid residues 30-43 of rat SSTR2 (Accession number P30680). Extracellular, N-terminus.
    Accession (Uniprot) Number P30680
    Gene ID 54305
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human, pig - 12/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.6 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat brain (lanes 1 and 3) and rat pancreas (lanes 2 and 4) lysates:
    1. Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006), (1:200).
    2. Anti-Somatostatin Receptor Type 2 (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of SSTR2 in rat hippocampus
    Immunohistochemical staining of rat hippocampus using Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006). A. SSTR2 appears in neural processes (green) that are perpendicular to dentate granule layer (arrow). B. Staining of axons with mouse anti Neurofilament 200 (NF200, red). C. Confocal merge of SSTR2 and NF200 images suggests that SSTR2 is not present in axons that run through the granule layer and hilus, but rather in neuronal dendrites that ascend toward the dentate hilus (arrow).
    Expression of SSTR2 in mouse cortex
    Immunohistochemical staining of frozen mouse cortex section using Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006), (1:100). A. SSTR2 appears in neural processes (green, horizontal arrow) that are perpendicular to the cortical surface (vertical arrow). B. Staining of axons with mouse anti-Neurofilament 200 (NF200, red) appears mostly in the deep layers. C. Confocal merge of SSTR2 and NF200 images suggests that SSTR2 is not present in axons that run in the deep layers, but rather in neuronal dendrites that ascend toward upper layers of cortex (vertical arrow).
    Live cell imaging / Immunocytochemistry
    Expression of SSTR2 in human HT-29 cells
    Immunocytochemical staining of SSTR2 in intact living human colorectal adenocarcinoma  (HT-29) cells. A. Extracellular staining of cells with Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006), (1:50), (red). B. Merged image of A with live view of cell.
    References
    1. Hofland, L.J. and Lamberts, S.W. (2001) Ann. Oncol. 12, S31.
    2. Fombonne, J. et al. (2003) Rep. Biol. Endocrinol1, 19.
    3. Slooter, G.D. et al. (2001) Br. J. Surg88, 31.
    4. Schulz, S. et al. (2002) Gyn. Oncol. 84, 235.
    Scientific background

    Somatostatin is a small cyclic peptide that is widely expressed throughout the central nervous system and peripheral tissues.1 In peripheral tissues, somatostatin exerts inhibitory effects on secretion processes, whereas in the brain, it acts as a neurotransmitter in both a stimulatory and an inhibitory manner.1,2
    Somatostatin mediates its action via six high affinity G-protein-coupled receptors (SSTR1, SSTR2a, SSTR2b, SSTR3SSTR4, and SSTR5), which are encoded by five genes.1,2 Expression of the different receptors is developmentally regulated in a time- and tissue-specific manner.2

    Somatostatin receptors have been found on a variety of neuroendocrine tumors, such as paragangliomas, carcinoids, and breast tumors.3 Synthetic peptide derivatives of somatostatin have been successfully used in the treatment of neuroendocrine malignancies and in vivo imaging of tumors that are positive to somatostatin receptors.4

    In general, SSTR2 is the most common SSTR subtype found in human tumors, followed by SSTR1, with SSTR3 and SSTR4 being less common.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 05/11/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against the N-terminus domain of the rat SSTR2. Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006), can be used in western blot, immunohistochemistry and immunocytochemistry appications. It has been designed to recognize SSTR2 from mouse, human and rat samples.

    For research purposes only, not for human use
    Citations
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