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Anti-TREM2 (extracellular) Antibody

Triggering receptor expressed on myeloid cells 2, Triggering receptor expressed on monocytes 2
Cat #: ANR-018
Alternative Name Triggering receptor expressed on myeloid cells 2, Triggering receptor expressed on monocytes 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KHWGRRKAW(S)RQLGE, corresponding to amino acid residues 42-56 of mouse TREM2 (Accession Q99NH8). Extracellular, N-terminus.
Accession (Uniprot) Number Q99NH8
Gene ID 83433
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – 14/15 amino acid residues identical; rat – 12/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Anti-TREM2 (extracellular) Antibody (#ANR-018), (1:200).
    3,4. Anti-TREM2 (extracellular) Antibody, preincubated with TREM2 (extracellular) Blocking Peptide (#BLP-NR018).
  • Western blot analysis of mouse BV-2 microglia (lanes 1 and 4), human U-87 MG glioblastoma (lanes 2 and 5) and human THP-1 monocytic leukemia (lanes 3 and 6) cell line lysates:
    Western blot analysis of mouse BV-2 microglia (lanes 1 and 4), human U-87 MG glioblastoma (lanes 2 and 5) and human THP-1 monocytic leukemia (lanes 3 and 6) cell line lysates:
    1-3. Anti-TREM2 (extracellular) Antibody (#ANR-018), (1:200).
    4-6. Anti-TREM2 (extracellular) Antibody, preincubated with TREM2 (extracellular) Blocking Peptide (#BLP-NR018).
Indirect flow cytometry
  • Cell surface detection of TREM2 in live intact mouse J774 macrophage cells:
    Cell surface detection of TREM2 in live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-TREM2 (extracellular) Antibody (#ANR-018), (2.5 µg) + goat-anti-rabbit-FITC.
References
  1. Shirotani, K. et al. (2019) Sci. Rep. 9, 7508.
  2. Del-Aguila, J.L. et al. (2019) Mol. Neurodegener. 14, 18.
  3. Zhong, L. et al. (2019) Nat. Commun. 10, 1365.
Scientific background

TREM2 (triggering receptor expressed on myeloid cells-2) is a type I transmembrane microglial surface receptor that is found to be expressed on myeloid cells including monocyte-derived dendritic cells, osteoclasts and bone-marrow derived macrophage. TREM2 protein transduces intracellular signals that regulate microglial functions such as cytokine production, migration, proliferation, phagocytosis, cell survival, synapse elimination, and compaction of amyloid plaques1,2.

The TREM2 structure includes an immunoglobulin-like extracellular domain responsible for ligand binding, a transmembrane region and a short cytoplasmatic tail. The protein also includes an ectodomain that binds anionic and zwitterionic lipids, apolipoproteins and lipoprotein particles2,3.

Studies show that TREM2 knockout mice display decreased levels of inflammatory cytokines. TREM2 has been shown to control two signaling pathways in the brain: regulation of phagocytosis and suppression of inflammatory reactivity. Nasu-Hakola disease, a rare and fatal disease, and polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy has been found to be linked to homozygous loss-of-function mutations in TREM2 protein1,2. Studies also show that variants of TREM2 are associated with Alzheimer’s disease2,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-TREM2 (extracellular) Antibody (#ANR-018) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. The antibody recognizes an extracellular epitope and can be used to detect the protein in living cells. It has been designed to recognize TREM2 from mouse, human, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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