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Anti-TRPC2 Antibody

Short transient receptor potential channel 2, Transient receptor protein 2, TRP2, mTrp2

Cat #: ACC-027
Alternative Name Short transient receptor potential channel 2, Transient receptor protein 2, TRP2, mTrp2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)KNRSGVRMFKDGD, corresponding to amino acid residues 12-24 of mouse TRPC2 (Accession Q9R244). Intracellular, N-terminus.
Accession (Uniprot) Number Q9R244
Gene ID 22064
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 12/13 amino acid residues identical.
RRID AB_2340897.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.9 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of mouse brain lysate:
    Western blot analysis of mouse brain lysate:
    1. Anti-TRPC2 Antibody (#ACC-027), (1:200).
    2. Anti-TRPC2 Antibody, preincubated with TRPC2 Blocking Peptide (#BLP-CC027).
References
  1. Birnbaumer, L. et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 15195.
  2. Vannier, B. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 2060.
  3. Liman, E.R. et al. (1999). Proc. Natl. Acad. Sci. U.S.A. 96, 5791.
  4. Jungnickel, M.K. et al. (2001) Nat. Cell Biol. 3, 499.
  5. Liman, E.R. and Corey, D.P. (1996) J. Neurosci. 16, 4625.
  6. Stowers, L. et al. (2002) Science 295, 1493.
Scientific background

TRPC (transient receptor potential cation) channels are the closest mammalian homologs of Drosophila TRP and TRP-like channels. TRPCs are rather nonselective Ca2+ permeable cation channels and affect cell functions through their ability to mediate Ca2+ entry into cells and their action to collapse the plasma membrane potentials1.

The mammalian genome codes for seven TRPCs of which TRPC2 is the largest with the most restricted pattern of expression and has several alternatively spliced variant2. Expressed in model cells, TRPC2 mediates both receptor- and store depletion-triggered Ca2+ 3. In human, TPRC2 is a pseudogene3.

Physiological roles for TRPC2 have been studied in mature sperm and the vomeronasal sensory system. In sperm, TRPC2 is activated by the sperm’s interaction with the oocyte’s zona pellucida, leading to entry of Ca2+ and activation of the acrosome reaction4. In the vomeronasal sensory organ (VNO), TRPC2 was found to constitute the transduction channel activated through signaling cascade initiated by the interaction of pheromones with V1R and V2R G protein-coupled receptors on the dendrites of the sensory neurons5. TRPC2-deficient mice display abnormal mating behavior, consistent with a role for this channel in pheromone signaling6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Anti-TRPC2 Antibody (#ACC-027) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize TRPC2 from rat and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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