Anti-VMAT1 Antibody

Vesicular monoamine transporter 1, SLC18A1, CGAT, VAT1
    Cat #: AMT-007
    Alternative Name Vesicular monoamine transporter 1, SLC18A1, CGAT, VAT1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • Peptide (C)EFKDSNSSLHRGPS, corresponding to amino acid residues 53-66 of rat VMAT1 (Accession Q01818). 1st luminal loop.
    • Anti-VMAT1 Antibody
    Accession (Uniprot) Number Q01818
    Gene ID 25693
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 12/14 amino acid residues identical.
    RRID AB_2756668.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Not recommended for human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Anti-VMAT1 Antibody
      Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
      1,2. Anti-VMAT1 Antibody (#AMT-007), (1:200).
      3,4. Anti-VMAT1 Antibody, preincubated with the negative control antigen.
    References
    1. Wimalasena, K. (2011) Med. Res. Rev. 31, 483.
    2. Lawal, H.O. et al. (2013) Mol. Aspects. Med. 34, 360.
    3. Richards, M. et al. (2006) Behav. Brain Funct. 2, 39.
    Scientific background

    Vesicular monoamine transporters (VMAT) are transmembrane proteins located on membranes of monoaminergic neurons and are responsible for the uptake of cytosolic monoamines into synaptic vesicles. They store neurotransmitter molecules in synaptic vesicles for additional cycles of release. They are acidic glycoproteins encoded by the SLC18A1 gene and transport neurotransmitters including dopamine, serotonin, adrenaline, noradrenaline, histamine and norepinephrine1,2. Its structure is composed of 12 transmembrane domains, the C and N-terminus are located in the cytosolic side of the vesicle. The glycosylation sites are located in the vesicular matrix on a loop between transmembrane domain I and II1.

    VMAT have an important role in sorting and releasing of neurotransmitters and in the adjustment of neuronal and endocrine informational output.  The transport into the storage organelle is dependent on a vacuolar ATPase-generated proton gradient in exchange for protons.

    Two monoamine transporters have been identified in rats and in human: VMAT1 and VMAT2. In humans, VMAT1 is preferentially expressed in large dense core vesicles of various neuroendocrine cells, including chromaffin and enterochromaffin cells.

    Several studies have suggested that VMAT1 has a strong connection to schizophrenia, bipolar disorder, and other anxiety related personality disorders, partially due to the fact that VMAT1 maps to chromosome 8p21.3, a locus with strong evidence for linkage with schizophrenia1,3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat vesicular monoamine transporter 1. Anti-VMAT1 Antibody (#AMT-007) can be used in western blot analysis. It has been designed to recognize VMAT1 from rat and mouse samples. The antibody is not recommended to be used on human samples.

    For research purposes only, not for human use
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