Overview
Type: Synthetic peptide
Form: Lyophilized powder
ATP1B3 (extracellular) Blocking Peptide (#BLP-NP013) is the original antigen used for immunization during Anti-ATP1B3 (extracellular) Antibody (#ANP-013) generation. The blocking peptide binds and ‘blocks’ Anti-ATP1B3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4):1, 2. Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (1:200).
3, 4. Anti-ATP1B3 (extracellular) Antibody, preincubated with ATP1B3 (extracellular) Blocking Peptide (#BLP-NT013)
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1, 2. Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (1:200).
3, 4. Anti-ATP1B3 (extracellular) Antibody, preincubated with ATP1B3 (extracellular) Blocking Peptide (#BLP-NT013)
Expression of ATP1B3 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ATP1B3 (extracellular) Antibody (#ANP-013), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the in rat hippocampal dentate gyrus region, revealed immunoreactivity (green) in neurons of the hilus (H, arrows) and the granule layer (G). B. Pre-incubation of the antibody with ATP1B3 (extracellular) Blocking Peptide (BLP-NP013), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)NDEVPKYRDQIPSPG, corresponding to amino acid residues 66 - 80 of human Beta 3 Na+/K+ ATPase (Accession P54709).
Accession (Uniprot) Number P54709
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
