ATP1B3 (extracellular) Blocking Peptide (#BLP-NP013) is the original antigen used for immunization during Anti-ATP1B3 (extracellular) Antibody (#ANP-013) generation. The blocking peptide binds and ‘blocks’ Anti-ATP1B3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4):
- Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
- Expression of ATP1B3 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ATP1B3 (extracellular) Antibody (#ANP-013), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the in rat hippocampal dentate gyrus region, revealed immunoreactivity (green) in neurons of the hilus (H, arrows) and the granule layer (G). B. Pre-incubation of the antibody with ATP1B3 (extracellular) Blocking Peptide (BLP-NP013), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)NDEVPKYRDQIPSPG, corresponding to amino acid residues 66 - 80 of human Beta 3 Na+/K+ ATPase (Accession P54709).