Anti-ATP1B3 (extracellular) Antibody

Sodium/Potassium-Transporting ATPase Subunit Beta-3, CD298, Beta 3 Na+/K+ ATPase
  • New
Cat #: ANP-013
Alternative Name Sodium/Potassium-Transporting ATPase Subunit Beta-3, CD298, Beta 3 Na+/K+ ATPase
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)NDEVPKYRDQIPSPG, corresponding to amino acid residues 66 - 80 of human Beta 3 Na+/K+ ATPase (Accession P54709). Extracellular, C-terminus.
    • Anti-ATP1B3 (extracellular) Antibody
    Accession (Uniprot) Number P54709
    Gene ID 483
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-ATP1B3 (extracellular) Antibody
      Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4):
      1, 2. Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (1:200).
      3, 4. Anti-ATP1B3 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-ATP1B3 (extracellular) Antibody
      Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
      1, 2. Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (1:200).
      3, 4. Anti-ATP1B3 (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-ATP1B3 (extracellular) Antibody
      Cell surface detection of ATP1B3 in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (2.5 µg) + goat-anti-rabbit-FITC.
    1. Malik, N. et al. (1998) Mamm. Genome 9, 136.
    2. Lingrel, J.B. (1992) J. Bioenerg. Biomembr. 24, 263.
    3. McDonough, A.A. et al. (1990) FASEB J. 4, 1598.
    4. Arystarkhova, E. and Sweadner, K.J. et al. (1997) J. Biol. Chem. 272, 22405.
    Scientific background

    Na+/K+ ATPase enzyme is composed of two subunits, a large catalytic subunit (α) and a smaller glycoprotein subunit (β). The subunits assemble into a heterodimer localized to the plasma membrane1. There are three α subunits and three β subunits expressed in a tissue- and developmental-specific manner2.

    The Na+/K+ ATPase β subunit is composed of 302-304 amino acids, depending of the species or tissue location. The polypeptide is a single pas transmembrane protein. The protein structure is primarily helical from the amino terminus until just before the first glycosylation site3.

    Beta 3 Na+/K+ ATPase (ATP1B3) is highly expressed in rat lung and testis. It is also present in liver and skeletal muscle4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 15/07/2020

    Anti-ATP1B3 (extracellular) Antibody (#ANP-013) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize ATP1B3 from rat, mouse, and human samples.

    For research purposes only, not for human use
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