Bestrophin-1 (extracellular) Blocking Peptide (#BLP-BC001) is the original antigen used for immunization during Anti-Bestrophin-1 (extracellular) Antibody (#ABC-001) generation. The blocking peptide binds and ‘blocks’ Anti-Bestrophin-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat lung (lanes 1 and 3) and rat eye (lanes 2 and 4) lysate:
- Expression of Bestrophin-1 in rat lungImmunohistochemical staining of paraffin embedded rat lung sections using Anti-Bestrophin-1 (extracellular) Antibody (#ABC-001), (1:100). BEST1 is expressed both in the respiratory epithelium (red arrows) and in vascular smooth muscle (black arrows). Hematoxilin is used as the counterstain.
- (C)NPNKDYPGHEMD, corresponding to amino acid residues 259-270 of mouse Bestrophin-1 (Accession O88870).