Anti-Bestrophin-1 (extracellular) Antibody

BEST1, Vitelliform macular dystrophy protein 2, VMD2, BMD1
    Cat #: ABC-001
    Alternative Name BEST1, Vitelliform macular dystrophy protein 2, VMD2, BMD1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)NPNKDYPGHEMD, corresponding to amino acid residues 259-270 of mouse Bestrophin-1 (Accession O88870). 3rd extracellular loop.
        Anti-Bestrophin-1 (extracellular) Antibody
    Accession (Uniprot) Number O88870
    Gene ID 24115
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 10/12 amino acid residues identical; human - 9/12 amino acid residues identical.
    RRID AB_10560217.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, ih, wb
    May also work in: ic*, ip*, lci*
      • Anti-Bestrophin-1 (extracellular) Antibody
        Western blot analysis of rat lung (lanes 1 and 3) and rat eye (lanes 2 and 4) lysate:
        1,2. Anti-Bestrophin-1 (extracellular) Antibody (#ABC-001), (1:200).
        3,4. Anti-Bestrophin-1 (extracellular) Antibody, preincubated with the control peptide antigen.
      • Anti-Bestrophin-1 (extracellular) Antibody
        Expression of Bestrophin-1 in rat lung
        Immunohistochemical staining of paraffin embedded rat lung sections using Anti-Bestrophin-1 (extracellular) Antibody (#ABC-001), (1:100). BEST1 is expressed both in the respiratory epithelium (red arrows) and in vascular smooth muscle (black arrows). Hematoxilin is used as the counterstain.
      • Anti-Bestrophin-1 (extracellular) Antibody
        Cell surface detection of Bestrophin-1 in live intact Jurkat (human T cell leukemia) cell line:
        ___ Control cells + goat-anti-rabbit-FITC.
        ___ Cells + Anti-Bestrophin-1 (extracellular) Antibody (#ABC-001)(1:20) + goat-anti-rabbit-FITC.
        The control antigen is not suitable for this application.
    References
      • Mammalian Cl- channels can be broadly classified into four different families: voltage-dependent Cl- channels (CLCs), the cystic fibrosis transmembrane conductance regulator (CFTR), ligand-gated Cl- channels (γ-aminobutyric acid (GABA)) and glycine channels) and Ca2+-activated Cl- channels (Bestrophin and Anoctamin channels).

        Bestrophins were first found by genetic linkage of human-Bestrophin-1 (hBest1) to a juvenile form of macular degeneration called Best vitelliform macular dystrophy (BVMD)1,2. BVMD is mainly electrophysiologically characterized by a decrease in the light peak and physiologically by the thinning of the retina layer which eventually leads to the loss of central vision3. To date Bestrophin 1-4 have been identified, although Bestrophin-3 and Bestrophin-4 have been observed only at the RNA level3. In addition, splice variants of some of these Ca2+-activated Cl- channels (CaCCs) have also been detected2,4,5. CaCCs are known to be involved in the regulation of olfaction, taste, phototransduction, and excitability in the nervous system. Recently, Bestrophin-1 was shown to be functionally expressed in astrocytes in both primary cell culture and in situ6. Bestrophin-1 is also detected in retina, brain, spinal cord and testes7.

        Two different topologies for Bestrophin-1 have been proposed. The first, the preferred structure, proposes that six hydrophobic domains span the membrane8, while the second suggests that there are only four membrane-spanning domains9. Bestrophin-1, along with its counterparts, is activated by intracellular Ca2+. A recent study demonstrated that Bestrophin-1 indeed binds Ca2+ and by mutating specific residues, showed which amino acid residues are essential for binding Ca2+ 10, providing additional evidence that Bestrophin-1 is activated by direct binding of Ca2+ to the channel11,12.

        Bestrophin-1 has been found to release glutamate from astrocytes and is located at microdomains near synapses13.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse Bestrophin-1. Anti-Bestrophin-1 (extracellular) Antibody (#ABC-001) can be used in western blot, immunohistochemistry and indirect flow cytometry applications. It has been designed to recognize bestrophin-1 channel from mouse, rat and human samples.

    For research purposes only, not for human use
    Citations
      • Rat uterus lysate (1:2000).
        Mijuškovic´, A. et al. (2015) Br. J. Pharmacol. 172, 3671.
      • Rat spinal cord and DRG lysates (1:200).
        Pineda-Farias, J.B. et al. (2015) Mol. Pain 11, 1.
      • Rat DRG lysate (1:400).
        Garcia, G. et al. (2014) Brain Res. 1579, 35.
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