BQ-3020

A Selective and Potent Endothelin B Receptor Agonist
    Cat #: SPE-200
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 2006 Da.
    Purity: ≥98% (HPLC)
    Effective concentration 1 pM -100 nM.
    Sequence LMDKEAVYFAHLDIIW.
    Modifications Leu1 = N-terminal Ac.
      • BQ-3020
    Molecular formula C96H140N20O25S1.
    CAS No.: 143113-45-5.
    Activity BQ-3020 is a selective and potent ET-B receptor agonist1.
      • Ekelund, U. et al. (1994) Br. J. Pharmacol. 112, 887.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Soluble in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
      • BQ-3020
        Alomone Labs BQ-3020 activates Endothelin B receptor-mediated Ca2+ mobilization in CHO-K1-mt Aequorin cells.
        Dose-response curve of normalized activation of the Endothelin B receptor-mediated Ca2+ mobilization, evoked by BQ-3020 (#SPE-200). Ca2+ response was detected by measuring aequorin-derived fluorescence following application of BQ-3020 and Endothelin-1 at different concentrations. The EC50 measured for BQ-3020 is 23 pM.
    References - Scientific background
      • BQ-3020 is a synthetic peptide that acts as a selective and potent ET-B (endothelin B) receptor agonist, a receptor that belongs to the G protein-coupled receptors (GPCR). BQ-3020 is a linear analog of the ET-B receptor ligand endothelin 1 (ET-1), with very close structural similarity to ET-1 compared to other agonists. Several studies have shown that locally active in vivo administration of BQ-3020 causes arterial constriction in healthy human blood vessels1.

        Endothelin receptors include two subtypes: ET-A and ET-B. They are widely distributed in vascular and nonvascular tissues. ET-B receptor has high equal affinity for all endothelin isopeptides2. ET-B receptors play an important role in regulating renal function and blood pressure and are expressed in sensory nerves. They are mainly present in medium- and large-sized cell bodies of human trigeminal ganglia3.

        Endothelin receptor agonists are being used as useful tools for the functional characterization of these receptors. Studies have shown that administering ET-B receptor agonist into the rat renal medulla, leads to natriuresis and diuresis via a mechanism that is NOS1-dependent4.

    Target ET-B receptor
    Net Peptide Content: 100%
    Last update: 01/07/2019

    BQ-3020 (#SPE-200) is a highly pure, synthetic, and biologically active peptide.

    For research purposes only, not for human use