Sarafotoxin S6b

SRTX-B, S6B, Sarafotoxin-B
A Non-Selective and Potent Agonist of Endothelin Receptors
    Cat #: SPE-160
    Alternative Name SRTX-B, S6B, Sarafotoxin-B
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 2564 Da.
    Purity: ≥98% (HPLC)
    Effective concentration 1 pM - 100 nM.
    Sequence CSCKDMTDKECLYFCHQDVIW.
    Modifications Disulfide bonds between Cys1-Cys15 and Cys3-Cys11.
    Structure
    • Sarafotoxin S6b
    Molecular formula C110H159N27O34S5.
    CAS No.: 116303-65-2.
    Activity Sarafotoxin S6b is an endothelin receptor agonist1,2.
    References-Activity
    1. Bax, W.A. et al. (1994) Br. J. Pharmacol. 113, 1471.
    2. Maggi, C.A. et al. (1990) Gen. Pharmacol. 21, 247.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    • Sarafotoxin S6b
      Alomone Labs Sarafotoxin S6b activates Endothelin-B receptor-mediated Ca2+ Luminescence in CHO-K1-mt Aequorin cells.
      Dose-response curve of normalized activation of Endothelin-B receptor-mediated Ca2+ mobilization evoked by Sarafotoxin S6b (#SPE-160). Ca2+ response was detected by measuring aequorin-derived fluorescence following application of Sarafotoxin S6b and Endothelin-1 at different concentrations. The EC50 measured for Sarafotoxin S6b is 43 pM.
    References - Scientific background
    1. Bax, W.A. et al. (1994) Br. J. Pharmacol. 113, 1471.
    2. Aumelas, A. et al. (1999) Eur. J. Biochem. 266, 977.
    3. Davenport, A.P. et al. (2016) Pharmacol. Rev. 68, 357.
    4. Saeki, T. et al. (1991) Biochem. Biophys. Res. Commun. 179, 286.
    Scientific background

    Sarafotoxin S6b is a non-selective potent endothelin receptor agonist designed from the Lys-2-Arg-1 dipeptide of the endothelin pro-sequence. Sarafotoxin S6b has the ability to induce contractions of the human isolated coronary artery1,2.

    Studies have shown that acute infusion of an ET-B receptor agonist into the rat renal medulla leads to natriuresis and diuresis via a mechanism that is NOS1-dependent3. Thus, endothelin receptors pharmacology play an important role in studying the function of the receptors.

    Endothelin receptors include two subtypes: ET-A and ET-B. They are widely distributed in vascular and nonvascular tissues. ETA receptors are predominantly expressed in peripheral tissues, especially in vascular smooth muscle tissues to mediate vasoconstriction. They are also detected in several different regions of the brain. ET-A receptor has high affinity for endothelin-1 and endothelin-2 and relatively low affinity for endothelin-3, while ET-B receptor has equally high affinity for all endothelin isopeptides4.

    Target Endothelin receptors
    Net Peptide Content: 100%
    Last update: 23/03/2020

    Sarafotoxin S6b (#SPE-160) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Shipping and Ordering information