Overview
Cat #: BLP-CC005
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005) and Guinea pig Anti-CaV1.3 (CACNA1D) Antibody (#AGP-061). Intended to be used as a negative control.
Applications: bl
Application key:
BL- Blocking
For research purposes only, not for human use
Applications
Applications
Blocking
- Western blot analysis of rat brain membranes:1. Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005), (1:200).
2. Anti-CaV1.3 (CACNA1D) Antibody, preincubated with Cav1.3/CACNA1D Blocking Peptide (#BLP-CC005). - Expression of CaV1.3 in rat DRGImmunohistochemical staining of adult rat dorsal root ganglion (DRG) with Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005). Staining appears in clusters of cells (green) but not in axons. Staining of neurofilament 200 demonstrates partial overlap with CaV1.3 in neuronal staining but not in axons (red).
- Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and human brain neuroblastoma cells (SH-SY5Y) (lanes 3 and 6):
1-3. Guinea pig Anti-CaV1.3 (CACNA1D) Antibody (#AGP-061), (1:200).
4-6. Guinea pig Anti-CaV1.3 (CACNA1D) Antibody, preincubated with Cav1.3/CACNA1D Blocking Peptide (#BLP-CC005).
Following a broad screen of secondary antibodies, the following were used for this application:
Western blot analysis:
#106-035-006 (Jackson ImmunoResearch)
#A7289 (Sigma) - Expression of CaV1.3 in rat DRGsImmunohistochemical staining of rat dorsal root ganglion (DRG) using Guinea pig Anti-CaV1.3 (CACNA1D) Antibody (#AGP-061), (1:200). A. CaV1.3 staining (red) is detected in some DRG cells (arrows). B. Nuclei staining using DAPI as the counterstain. C. Merged image of panels A and B.
Properties
Properties
Sequence
Accession (Uniprot) Number P27732
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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