Cav1.3/CACNA1D Blocking Peptide (#BLP-CC005) is the original antigen used for immunization during Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005) generation. The blocking peptide binds and ‘blocks’ Anti-Cav1.3/CACNA1D primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of CaV1.3 in rat DRGImmunohistochemical staining of adult rat dorsal root ganglion (DRG) with Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005). Staining appears in clusters of cells (green) but not in axons. Staining of neurofilament 200 demonstrates partial overlap with CaV1.3 in neuronal staining but not in axons (red).
- Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and human brain neuroblastoma cells (SH-SY5Y) (lanes 3 and 6):
1-3. Guinea pig Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005-GP), (1:200).
4-6. Guinea pig Anti-CaV1.3 (CACNA1D) Antibody, preincubated with Cav1.3/CACNA1D Blocking Peptide (#BLP-CC005).
Following a broad screen of secondary antibodies, the following were used for this application:
Western blot analysis:
#106-035-006 (Jackson ImmunoResearch)
- Expression of CaV1.3 in rat DRGsImmunohistochemical staining of rat dorsal root ganglion (DRG) using Guinea pig Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005-GP), (1:200). A. CaV1.3 staining (red) is detected in some DRG cells (arrows). B. Nuclei staining using DAPI as the counterstain. C. Merged image of panels A and B.
- (C)DNKVTIDDYQEEAEDKD, corresponding to amino acid residues 859-875 of rat CaV1.3 (Accession P27732).