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Anti-CaV1.3 (CACNA1D) Antibody

Voltage-dependent L-type calcium channel subunit α1D
Cat #: ACC-005
Alternative Name Voltage-dependent L-type calcium channel subunit α1D
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)DNKVTIDDYQEEAEDKD, corresponding to amino acid residues 859-875 of rat CaV1.3 (Accession P27732). Intracellular loop between domains II and III.
    Accession (Uniprot) Number P27732
    Gene ID 29716
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Hamster, mouse - 16/17 amino acid residues identical; human -15/17 amino acid residues identical; chicken - 12/17 amino acid residues identical.
    RRID AB_2039775.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
    Western blot
    • Western blot analysis of rat brain membranes:
      Western blot analysis of rat brain membranes:
      1. Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005), (1:200).
      2. Anti-CaV1.3 (CACNA1D) Antibody, preincubated with Cav1.3/CACNA1D Blocking Peptide (#BLP-CC005).
    • Human SAN tissue (Le Scouarnec, S. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105, 15617.).
    Immunoprecipitation
    • Rat cerebral cortex, thalamus, cerebellum, hippocampus, and spinal cord lysates (Kim, S. et al. (2007) J. Biol. Chem. 282, 32877.).
    Immunohistochemistry
    • Expression of CaV1.3 in rat DRG
      Expression of CaV1.3 in rat DRG
      Immunohistochemical staining of adult rat dorsal root ganglion (DRG) with Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005). Staining appears in clusters of cells (green) but not in axons. Staining of neurofilament 200 demonstrates partial overlap with CaV1.3 in neuronal staining but not in axons (red).
    Immunocytochemistry
    • Mouse SAN cells (Le Scouarnec, S. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105, 15617.).
    References
    1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
    2. IUPHAR
    3. Mangoni, M.E. et al (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 5543.
    4. Zhang, Z. et al. (2005) Circulation 112, 1936.
    Scientific background

    All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous.1,2

    The CaV1.3 channels are also expressed, as are other L-type channels, in neurons and neuroendocrine cells. However, accumulated data has shown the expression of CaV1.3 in heart and suggests that it plays a major role in the generation of cardiac pacemaker activity.3,4

    Several peptidyl toxins have been described that are specific L-type channel blockers. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700). So far no selective blocker for one of the CaV1 isoforms has been described.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-CaV1.3 (CACNA1D) Antibody
    Knockout validation of Anti-CaV1.3 (CACNA1D) Antibody in mouse eye sections.Immunohistochemical staining of mouse eye sections using Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005). CaV1.3 immunoreactivity (green) is detected in major retinal layers (upper panel). CaV1.3 is not detected in CaV1.3-/- mice.Adapted from Shi, L. et al. (2017) Front. Cell. Neurosci. 11, 232. with permission of Frontiers.

    Last update: 30/09/2020

    Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CaV1.3 from mouse, rat, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Published figures using this product
    • Expression of CaV1.3 in DA neurons from SNc and VTA.
      Expression of CaV1.3 in DA neurons from SNc and VTA.
      Immunohistochemical staining of rat brain sections using Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003) or Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005), shows that CaV1.3 channel (green) is detected in SNc and VTA regions while little or no CaV1.2 is expressed. CaV1.3 co-localizes with TH (red) in the soma as well as in the proximal dendrites of SNc and VTA DA neurons.
      Adapted from Philippart, F. et al. with permission of the Society for Neuroscience.
    • Expression of CaV α subunits in rat ICG.
      Expression of CaV α subunits in rat ICG.
      Immunohistochemical staining of rat intracardiac ganglia (ICG) using Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003), Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005), Anti-CACNA1A (CaV2.1) Antibody (#ACC-001), Anti-CACNA1B (CaV2.2) Antibody (#ACC-002) and Anti-CaV2.3 (CACNA1E) Antibody (#ACC-006). All CaV subtypes are expressed in sham treated rats but N-type CaV channel levels are decreased in CHF rats.
      Adapted from Tu, H. et al. (2014) with permission of the American Physiological Society.
    • Expression of CaV1.3 in mouse cochlea.
      Expression of CaV1.3 in mouse cochlea.
      Immunohistochemical staining of mouse cochlear sections using Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005). CaV1.3 staining (red) is detected in both apical (upper panels) and basal (lower panels) regions of the cochlea. Neurons were labeled with Tuj1 (green) and nuclei were stained with DAPI (blue). Scale bar, 20 μm.
      Adapted from Lv, P. et al. (2014) with permission of the Society for Neuroscience.
    KO validation citations
    1. Immunohistochemical staining of mouse retina sections. Tested in CaV1.3-/- mice.
      Shi, L. et al. (2017) Front. Cell. Neurosci. 11, 232.
    Western blot citations
    1. Human SH-SY5Y cell lysate and mouse brain lysate.
      Sun, Y. et al. (2017) J. Neurosci. 37, 3364.
    2. Mouse cochlea lysate (1:200).
      Vincent, P.F. et al. (2017) J. Neurosci. 37, 2960.
    3. Mouse astrocyte lysate (1:1000).
      Cheli, V.T. et al. (2016) Glia 64, 1396.
    4. Mouse heart lysate.
      Walton, R.D. et al. (2016) J. Gerontol. A Biol. Sci. Med. Sci. 71, 1005.
    5. Mouse eye lysate.
      Grabner, C.P. et al. (2015) J. Neurosci. 35, 13133.
    6. Rat retinae lysate (1:300).
      Grimes, W.N. et al. (2015) J. Neurophysiol. 114, 341.
    7. Rat heart lysates (1:1000).
      Liao, P. et al. (2015) J. Biol. Chem. 290, 9262.
    8. Rat brain lysates (1:200).
      N’Gouemo, P. et al. (2015) Int. J. Neuropsychopharmacol. 18, pyu123.
    9. Avian retinal lysates.
      Huang, C.C. et al. (2013) PLoS ONE 8, e73315.
    10. Chick retina lysate.
      Ko, M.L. et al. (2013) J. Neurochem. 127, 314.
    11. Mouse mesenteric and pulmonary arteries (1:200).
      Ko, E.A. et al. (2013) Am. J. Physiol. 304, C1042.
    12. Rat primary submucosa cells.
      Rehn, M. et al. (2013) Cell Tissue Res. 353, 355.
    13. Human SAN tissue.
      Le Scouarnec, S. et al. (2008) Proc. Natl. Acad. Sci. 105, 15617.
    Immunoprecipitation citations
    1. Rat retinae lysate (1-2 µg / 500 µg protein).
      Grimes, W.N. et al. (2015) J. Neurophysiol. 114, 341.
    2. Transfected HeK 293 cells.
      Reichhart, N. et al. (2015) Cell. Signal. 27, 2231.
    3. Rat cerebral cortex, thalamus, cerebellum, hippocampus, and spinal cord lysates.
      Kim, S. et al. (2007) J. Biol. Chem. 282, 32877.
    Immunohistochemistry citations
    1. Mouse retina sections. Also tested in CaV1.3-/- mice.
      Shi, L. et al. (2017) Front. Cell. Neurosci. 11, 232.
    2. Mouse cochlea sections.
      Vincent, P.F. et al. (2017) J. Neurosci. 37, 2960.
    3. Mouse inner ear sections (1:75).
      Ohn, T.L. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, E4716.
    4. Rat brain sections (1:100).
      Philippart, F. et al. (2016) J. Neurosci. 36, 7234.
    5. Mouse brain sections (1:200).
      Blosa, M. et al. (2015) J. Physiol. 593, 4341.
    6. Mouse eye sections.
      Grabner, C.P. et al. (2015) J. Neurosci. 35, 13133.
    7. Mouse cochlear sections (1:50).
      Jung, S. et al. (2015) Proc. Natl. Acad. Sci. U.S.A. 112, E3141.
    8. Mouse brain sections (1:200).
      Kim, Y.S. et al. (2015) Eur. J. Neurosci. 42, 2467.
    9. Mouse eye sections (1:100).
      Reichhart, N. et al. (2015) Cell. Signal. 27, 2231.
    10. Mouse cochlear sections.
      Lv, P. et al. (2014) J. Neurosci. 34, 7383.
    11. Mouse cochlea.
      Pirone, A. et al. (2014) J. Neurosci. 34, 434.
    12. Rat intracardiac ganglia and stellate ganglia neurons.
      Tu, H. et al. (2014) Am. J. Physiol. 306, C132.
    13. Human and mouse fetal lung sections (1:50 - 1:100).
      Brennan, S.C. et al. (2013) PLoS ONE 8, e80294.
    14. Human adrenal cortex sections (1:100-1:250).
      Scholl, U.I. et al. (2013) Nat. Genet. 45, 1050.
    15. Mouse apical cochlear turns (1:75).
      Wong, A.B. et al. (2013) J. Neurosci. 33, 10661.
    Immunocytochemistry citations
    1. Mouse astrocytes (1:200).
      Cheli, V.T. et al. (2016) Glia 64, 1396.
    2. Mouse spinal ganglion neurons (SGNs) (1:100-1:500).
      Lv, P. et al. (2014) J. Neurosci. 34, 7383.
    3. Rat pinealocytes.
      Mizutani, H. et al. (2014) Am. J. Physiol. 306, C1008.
    4. Avian cultured retinas (1:100).
      Huang, C.C. et al. (2013) PLoS ONE 8, e73315.
    5. Rat primary submucosa cells (1:50).
      Rehn, M. et al. (2013) Cell Tissue Res. 353, 355.
    6. Rat SCG and hippocampal pyramidal neurons (1:1000).
      Wheeler, D.G. et al. (2012) Cell 149, 1112.
    7. Mouse SAN cells.
      Le Scouarnec, S. et al. (2008) Proc. Natl. Acad. Sci. 105, 15617.
    More product citations
    1. Jing, Z. et al. (2013) J. Neurosci. 33, 4456.
    2. Johnson, S.L. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 8720.
    3. Reinbothe, T.M. et al. (2013) Diabetologia 56, 340.
    4. Xie, L. et al. (2013) Traffic 14, 428.
    5. Christel, C.J. et al. (2012) J. Physiol. 590, 6327.
    6. Lv, P. et al. (2012) J. Neurosci. 32, 16314.
    7. Walter, H.J. et al. (2000) J. Biol. Chem. 275, 25717.
    8. Bae, I.H. et al. (1999) Korean J. Biol. Sci. 3, 53.
    9. Hernandez, M.E. et al. (1999) Neuroendocrinology 70, 31.
    10. Jiang, Z. et al. (1999) Eur. J. Neurosci. 11, 3481.
    11. Lopez, I. et al. (1999) Neuroscience 92, 773.
    12. Serrano, C.J. et al. (1999) FEBS Letters 462, 171.
    13. Yang, S.N. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 10164.
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