Connexin-37 Blocking Peptide (#BLP-CC204) is the original antigen used for immunization during Anti-Connexin-37 Antibody (#ACC-204) generation. The blocking peptide binds and ‘blocks’ Anti-Connexin-37 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse heart lysate (lanes 1 and 4), rat lung membranes (lanes 2 and 5) and mouse lung lysate (lanes 3 and 6):
- Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate:
- (C)EHQMAKISVAEDGR, corresponding to amino acid residues 131-144 of rat Connexin-37 (Accession Q03190).