Anti-Connexin-37 Antibody

CX37, Gap junction α4 protein, GJA4
Cat #: ACC-204
Alternative Name CX37, Gap junction α4 protein, GJA4
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)EHQMAKISVAEDGR, corresponding to amino acid residues 131-144 of rat Connexin-37 (Accession Q03190). Intracellular loop.
    Accession (Uniprot) Number Q03190
    Gene ID 25655
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human – 13/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic, ifc, ih, ip
      • Western blot analysis of mouse heart lysate (lanes 1 and 4), rat lung membranes (lanes 2 and 5) and mouse lung lysate (lanes 3 and 6):
        1-3. Anti-Connexin-37 Antibody (#ACC-204), (1:400).
        4-6. Anti-Connexin-37 Antibody, preincubated with the control peptide antigen.
        Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate:
        1. Anti-Connexin-37 Antibody (#ACC-204), (1:200).
        2. Anti-Connexin-37 Antibody, preincubated with the control peptide antigen.
      • Meens, M.J. et al. (20120) Swiss Med. Wkly. 142, w13553.
      • Good, M.E. et al. (2014) J. Biol. Chem. 289, 30379.
      • Pogoda, K. et al. (2014) Cell. Commun. Signal. 12, 33.
      • Li, Y.Y. et al. (2013) Biomed. Rep. 1, 464.
      • Connexins (Cxs) are a family of transmembrane proteins comprising 21 members in humans that forms gap junction channels (GJCs). Connexins are divided in four groups: α, β, γ and δ. Cx37 is also named α4 since it is the fourth connexin of the alpha group that was identified1,2

        Connexins do not function as single proteins; six connexins assemble into connexons (hemi-channels), and two connexons from neighboring cells dock in the extracellular space to form a full gap junction channel. Connexins are responsible for mediating direct communication between adjacent cells by the exchange of ions and small signaling molecules and cytosolic factors1,3. Their structure consists of four transmembrane helices, two extracellular loops, a cytoplasmic loop, a cytoplasmic NH2- and COOH-termini1,2.

        Connexin-37 is encoded by the GJA4 gene and is expressed in vascular endothelial cells. The protein is responsible for regulating and controlling the growth, proliferation and regeneration of the vascular endothelium by mediating the signal transduction between smooth muscle and endothelial cells. Cx37 knockout mice have a tendency for developing endarterium atheromatous plaques4. Also, a polymorphism in the gene that encodes for Cx37, is associated with increased risk for atherosclerosis and myocardial infarction1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 07/04/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Connexin-37. Anti-Connexin-37 Antibody (#ACC-204) can be used in western blot analysis. It has been designed to recognize Cx37 from rat, mouse and human samples.

    For research purposes only, not for human use