Connexin-45/GJC1 Blocking Peptide (#BLP-CC207) is the original antigen used for immunization during Anti-Connexin-45 (GJC1) Antibody (#ACC-207) generation. The blocking peptide binds and ‘blocks’ Anti-Connexin-45/GJC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
- Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:
- Expression of Connexin-45 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Connexin-45 (GJC1) Antibody (#ACC-207), (1:400), followed by anti-rabbit-Cy3 antibody. Cx45 staining (red) appears in the neuropil of layers stratum oriens and stratum radiatum but not in the pyramidal layer. Nuclei are stained with DAPI (blue).
- (C)KEQSQPKPKHDGRR, corresponding to amino acid residues 153-166 of rat Connexin-45 (Accession A4GG66).