Every lot is tried & tested in a relevant biological assay.
- Behrendt, H.J. et al. (2004) Br. J. Pharmacol. 141, 737.
- Alomone Labs Frescolat MGA activates TRPM8 channels expressed in HEK-293 cells.Dose response curve of the activation of TRPM8, shown as relative fluorescence plotted against increasing concentrations of Frescolat MGA (#F-165). EC50 was determined at 1.77 µM. Cells were loaded with Calcium-5 dye. Changes in intracellular Ca2+ following agonist application were detected as changes in 485 nm/525 nm fluorescence (RFU).
Frescolat MGA is a synthetic compound that acts as a TRPM8 channel activator. Studies show that Frescolat MGA has the ability to activate mouse TRPM8 channels and to produce concentration-dependent increase in Ca2+ levels with an EC50 value of 4.8 µM1.
TRPM8 channel is a member of the TRP channels superfamily. TRPM8 is Ca2+-permeable, nonselective cation channel that is activated by temperatures in the range of 8–28°C and by chemical agonists such as menthol and icilin. TRPM8 is specifically expressed in temperature-sensing trigeminal and dorsal root ganglion neurons and is up-regulated in prostate cancer and other malignancies1,2.
Frescolat MGA (#F-165) is a highly pure, synthetic, and biologically active compound.