Name: FTX-3.3
Brand: Alomone Labs
SKU: F-120

Cat #: F-120

Alternative Name Funnel Web Spider Toxin

Lyophilized Powder yes

Bioassay Tested

Origin Agelenopsis aperta (North American funnel-web spider).

Source Synthetic

MW: 273.4

Purity: >99%

Effective concentration 0.1-1 mM.

Structure

Chemical name 1, 4-Dihydro-2,6-dimethyl-4-(2-nitrophenyl)-3,5-pyridinedicarboxylic acid dimethyl ester.

Molecular formula C₁₂H₃₁N₇.

Activity FTX-3.3 is a synthetic polyamine^1-2, reputed to be structurally identical to the native Funnel Web Spider Toxin (FTX). It blocks P-, N-, and L-type Ca²⁺ channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies³.

References-Activity

Blagbrough, I.S. et al. (1994) Tetra. Lett. 35, 2057.
Moya, E. et al. (1994) Tetra. Lett. 35, 2061.
Norris, T.M. et al. (1996) Mol. Pharmacol. 50, 939.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions Up to four weeks at 4°C or three months at -20°C.

Our bioassay

Alomone Labs FTX-3.3 blocks P-type Ca²⁺ currents in Xenopus oocytes.
A. Time course of P-type channel (Ca_V2.1+α2δ1+β1a) activity before and during applications of 500 mM FTX-3.3 (#F-120), and upon wash. Holding potential was -80 mV and currents were elicited every 10 seconds by 100 ms step to 0 mV. B. Superimposed current traces of P-type channels, before and during applications of 500 μM FTX-3.3 (taken from the experiment described in A).

References - Scientific background

Llinas, R. et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 85, 1689.
Llinas, R. et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 85, 1689.
Scott, R.H. et al. (1992) Br. J. Pharmacol. 106, 199.
Blagbrough, I.S. et al. (1994) Tetra. Lett. 35, 2057.
Moya, E. et al. (1994) Tetra. Lett. 35, 2061.
Dupere, J.R.B. et al. (1996) Neuropharmacology 35, 1.
Norris, T.M. et al. (1996) Mol. Pharmacol. 50, 939.
Fatehi, M. et al. (1997) Neuropharmacology 36, 185.

Scientific background

FTX is a polyamine component of the venom of the American funnel web spider Agelenopsis aperta, and has been described as a selective antagonist of P-type voltage-dependent Ca²⁺ channels^1-2. However, it has been demonstrated that FTX has actions at other sites including NMDA and GABA receptors and also on T-type calcium channels³.

FTX-3.3 (reputed to be structurally identical to the native FTX) has been synthesized^4-5 and shown to block P-type calcium channels in rat cerebellar Purkinje cells⁶. It antagonizes P-, N-, and L-type calcium channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies; Its IC₅₀ against P-, N-, and L-type channels are ~0.13 mM, ~0.24 mM and ~0.24 mM, respectively⁷.

FTX-3.3 does not reduce whole-cell sodium current recorded from SK.N.SH cells and is therefore ion selective⁸.

Target P-type Ca²⁺ channels

Lyophilized Powder

Last update: 13/10/2022

FTX-3.3 (#F-120) is a highly pure, synthetic, and biologically active compound.

Order Summary

FTX-3.3

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