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FTX-3.3

Funnel Web Spider Toxin

A Blocker of P-Type CaV Channels

Cat #: F-120
Alternative Name Funnel Web Spider Toxin
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Agelenopsis aperta (North American funnel-web spider).
    Source Synthetic
    MW: 273.4
    Purity: >99%
    Effective concentration 0.1-1 mM.
    Structure
    Chemical name 1, 4-Dihydro-2,6-dimethyl-4-(2-nitrophenyl)-3,5-pyridinedicarboxylic acid dimethyl ester.
    Molecular formula C12H31N7.
    Activity FTX-3.3 is a synthetic polyamine1-2, reputed to be structurally identical to the native Funnel Web Spider Toxin (FTX). It blocks P-, N-, and L-type Ca2+ channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies3.
    References-Activity
    1. Blagbrough, I.S. et al. (1994) Tetra. Lett. 35, 2057.
    2. Moya, E. et al. (1994) Tetra. Lett. 35, 2061.
    3. Norris, T.M. et al. (1996) Mol. Pharmacol. 50, 939.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs FTX-3.3 blocks P-type Ca2+ currents in Xenopus oocytes.
      Alomone Labs FTX-3.3 blocks P-type Ca2+ currents in Xenopus oocytes.
      A. Time course of P-type channel (CaV2.1+α2δ1+β1a) activity before and during applications of 500 mM FTX-3.3 (#F-120), and upon wash. Holding potential was -80 mV and currents were elicited every 10 seconds by 100 ms step to 0 mV. B. Superimposed current traces  of P-type channels, before and during applications of 500 μM FTX-3.3 (taken from the experiment described in A).
    References - Scientific background
    1. Llinas, R. et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 85, 1689.
    2. Llinas, R. et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 85, 1689.
    3. Scott, R.H. et al. (1992) Br. J. Pharmacol. 106, 199.
    4. Blagbrough, I.S. et al. (1994) Tetra. Lett. 35, 2057.
    5. Moya, E. et al. (1994) Tetra. Lett. 35, 2061.
    6. Dupere, J.R.B. et al. (1996) Neuropharmacology 35, 1.
    7. Norris, T.M. et al. (1996) Mol. Pharmacol. 50, 939.
    8. Fatehi, M. et al. (1997) Neuropharmacology 36, 185.
    Scientific background

    FTX is a polyamine component of the venom of the American funnel web spider Agelenopsis aperta, and has been described as a selective antagonist of P-type voltage-dependent Ca2+ channels1-2. However, it has been demonstrated that FTX has actions at other sites including NMDA and GABA receptors and also on T-type calcium channels3.

    FTX-3.3 (reputed to be structurally identical to the native FTX) has been synthesized4-5 and shown to block P-type calcium channels in rat cerebellar Purkinje cells6. It antagonizes P-, N-, and L-type calcium channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies; Its IC50 against P-, N-, and L-type channels are ~0.13 mM, ~0.24 mM and ~0.24 mM, respectively7.

    FTX-3.3 does not reduce whole-cell sodium current recorded from SK.N.SH cells and is therefore ion selective8.

    Target P-type Ca2+ channels
    Last update: 02/01/2022

    FTX-3.3 (#F-120) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

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