Name: sFTX-3.3
Brand: Alomone Labs
SKU: F-130

Cat #: F-130

Alternative Name Synthetic Funnel Web Spider Toxin, Arginyl polyamine

Lyophilized Powder yes

Bioassay Tested

Source Synthetic

MW: 287.4

Purity: >95%

Effective concentration 0.1-1 mM.

Structure

Chemical name Pentanamide,2-amino-5-[(aminoiminomethyl)amino]-N-[3-[(3-aminopropyl)amino]propyl]-, (2S)-.

Molecular formula C₁₂H₂₉N₇O.

CAS No.: 141997-14-0

Activity sFTX-3.3, a synthetic funnel web spider toxin^1-2, is a polyamine amide analogue of FTX. It blocks P-, N-, and L-type Ca²⁺ channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies³.

References-Activity

Blagbrough, I.S. et al. (1994) Tetra. Lett. 35, 2057.
Moya, E. et al. (1994) Tetra. Lett. 35, 2061.
Norris, T.M. et al. (1996) Mol. Pharmacol. 50, 939.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions Up to four weeks at 4°C or three months at -20°C.

Our bioassay

Alomone Labs sFTX-3.3 blocks P-type Ca²⁺ currents in Xenopus oocytes.
A. Time course of P-type channel (Ca_V2.1+a2d1+b1a) activity before and during applications of 500 μM sFTX-3.3 (#F-130), and upon wash. Holding potential was -80 mV and currents were elicited every 10 seconds by 100 ms step to 0 mV. B. Superimposed current traces of P-type channels, before and during applications of 500 μM sFTX-3.3 (taken from the experiment described in A).

References - Scientific background

Cherksey, B.D. et al. (1991) Ann. N. Y. Acad Sci. 635, 80.
Sutton, K.G. et al. (197) Comp. Biochem. Physiol. 116C, 23.
Llinas, R. et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 85, 1689.
Lin, J.W. et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 4538.
Scott, R.H. et al. (1992) Br. J. Pharmacol. 106, 199.
Blagbrough, I.S. et al. (1994) Tetra. Lett. 35, 2057.
Moya, E. et al. (1994) Tetra. Lett. 35, 2061.
Dupere, J.R.B. et al. (1996) Neuropharmacology 35, 1.
Norris, T.M. et al. (1996) Mol. Pharmacol. 50, 939.

Scientific background

Polyamine amide spider toxins inhibit neuronal voltage-activated Ca²⁺ currents. A terminal arginine group is an important feature of these Ca²⁺ channel antagonists^1-2.

FTX is a polyamine component of the venom of the American funnel web spider Agelenopsis aperta, and has been described as a selective antagonist of P-type voltage-dependent Ca²⁺ channels^3-4. However, it has been demonstrated that FTX has actions at other sites including NMDA and GABA receptors and also on T-type calcium channels⁵.

sFTX-3.3, a synthetic funnel web spider toxin^6-7, is a polyamine amide analogue of FTX. The structures of these polyamine containing compounds are not identical: sFTX-3.3 contains an amide carbonyl oxygen that is absent from the predicted structure of native FTX⁸. sFTX-3.3 has been shown to block P-type calcium channels in rat cerebellar Purkinje cells⁸ and antagonizes P-, N-, and L-type calcium channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies. Its IC₅₀ against P-, N-, and L-type channels are ~0.24 mM, ~0.7 mM and ~0.7 mM, respectively⁹.

Target P-type Ca²⁺ channels

Lyophilized Powder

Last update: 13/10/2022

sFTX-3.3 (#F-130) is a highly pure, synthetic, and biologically active compound.

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sFTX-3.3

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