Overview
Type: Synthetic peptide
Form: Lyophilized powder
GRIK3/GluK3 (extracellular) Blocking Peptide (#BLP-GC040) is the original antigen used for immunization during Anti-GRIK3 (GluK3) (extracellular) Antibody (#AGC-040) generation. The blocking peptide binds and ‘blocks’ Anti-GRIK3/GluK3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-GRIK3 (GluK3) (extracellular) Antibody (#AGC-040), (1:200).
3,4. Anti-GRIK3 (GluK3) (extracellular) Antibody, preincubated with GRIK3/GluK3 (extracellular) Blocking Peptide (#BLP-GC040).
Expression of GluR7 in mouse hippocampus and rat cerebellumImmunohistochemical staining of immersion-fixed, free floating frozen brain sections using Anti-GRIK3 (GluK3) (extracellular) Antibody (#AGC-040), (1:400). A. GluR7 staining (red) in mouse hippocampus reveals expression in the pyramidal (P) layer (arrow). B. GluR7 staining (red) in rat cerebellum reveals expression in the Purkinje (P) layer (horizontal arrow) and in the molecular layer (MOL) interneurons (arrow).
Properties
Sequence
- (C)DGPNAQVMNAEEH, corresponding to amino acid residues 46-58 of rat kainate receptor GluK3 (Accession P42264).
Accession (Uniprot) Number P42264
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
