GRIK3/GluK3 (extracellular) Blocking Peptide (#BLP-GC040) is the original antigen used for immunization during Anti-GRIK3 (GluK3) (extracellular) Antibody (#AGC-040) generation. The blocking peptide binds and ‘blocks’ Anti-GRIK3/GluK3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
- Expression of GluR7 in mouse hippocampus and rat cerebellumImmunohistochemical staining of immersion-fixed, free floating frozen brain sections using Anti-GRIK3 (GluK3) (extracellular) Antibody (#AGC-040), (1:400). A. GluR7 staining (red) in mouse hippocampus reveals expression in the pyramidal (P) layer (arrow). B. GluR7 staining (red) in rat cerebellum reveals expression in the Purkinje (P) layer (horizontal arrow) and in the molecular layer (MOL) interneurons (arrow).
- (C)DGPNAQVMNAEEH, corresponding to amino acid residues 46-58 of rat kainate receptor GluK3 (Accession P42264).