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Anti-GRIK3 (GluK3) (extracellular) Antibody

Glutamate receptor ionotropic kainate 3, Glutamate receptor 7, GluR7

Cat #: AGC-040
Alternative Name Glutamate receptor ionotropic kainate 3, Glutamate receptor 7, GluR7
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DGPNAQVMNAEEH, corresponding to amino acid residues 46-58 of rat kainate receptor GluK3 (Accession P42264). Extracellular, N-terminus.
Accession (Uniprot) Number P42264
Gene ID 298521
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, mouse - identical.
RRID AB_2340955.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    1,2. Anti-GRIK3 (GluK3) (extracellular) Antibody (#AGC-040), (1:200).
    3,4. Anti-GRIK3 (GluK3) (extracellular) Antibody, preincubated with GRIK3/GluK3 (extracellular) Blocking Peptide (#BLP-GC040).
Immunohistochemistry
  • Expression of GluR7 in mouse hippocampus and rat cerebellum
    Expression of GluR7 in mouse hippocampus and rat cerebellum
    Immunohistochemical staining of immersion-fixed, free floating frozen brain sections using Anti-GRIK3 (GluK3) (extracellular) Antibody (#AGC-040), (1:400). A. GluR7 staining (red) in mouse hippocampus reveals expression in the pyramidal (P) layer (arrow). B. GluR7 staining (red) in rat cerebellum reveals expression in the Purkinje (P) layer (horizontal arrow) and in the molecular layer (MOL) interneurons (arrow).
References
  1. Perrais, D. et al. (2009) Neuropharmacology 56,131
  2. Mayer, M.L. (2011) Curr. Opin. Neurobiol. 21, 283.
  3. Fernández-Alacid, L. et al. (2011) Eur. J. Neurosci. 34, 1724.
  4. Lerma, J. and Marques, J.M. (2013) Neuron 80, 292.
  5. Begni, S. et al. (2002) Mol. Psychiatry 7, 416.
  6. Schiffer, H.H. and Heinemann S.F. (2007) Am. J. Med. Genet. B. Neuropsychiatr. Genet. 144B, 20.
Scientific background

Ionotropic glutamate receptors (iGluRs) mediate the majority of fast excitatory synaptic neurotransmission in the central nervous system. The selective assembly of iGluRs into AMPAkainate, and N-methyl-D-aspartic acid (NMDA) receptor subtypes is regulated by their extracellular amino-terminal domains (ATDs)1.

Kainate receptors (KARs) are tetramers classified into low-affinity receptor families (GluK1–GluK3) and high-affinity receptor families (GluK4–GluK5) based on their affinity for the neurotoxin kainic acid. KARs share a similar architecture with other ionotropic glutamate receptors; the subunits have a large extracellular domain composed of an amino-terminal domain (ATD) and a ligand binding domain (LBD) and an intracellular carboxy-terminal region2.

KARs are expressed in neurons and glial cells throughout the CNS. GluK3 is poorly expressed, appearing in layer IV of the neocortex and dentate gyrus in the hippocampus3.

KARs serve a crucial role as modulators of synaptic transmission and plasticity and their dysfunction has been linked to several disease states such as epilepsy, chronic pain and neurodegenerative diseases4. Alteration in the extracellular domain of GluK3, is in linkage disequilibrium with recurrent major depressive disorder patients5 and subjects with schizophrenia6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 25/07/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat kainate receptor GluK3. Anti-GRIK3 (GluK3) (extracellular) Antibody (#AGC-040) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus highly suited to recognize the receptor in living cells. It has been designed to recognize GluR7 from human, rat and mouse samples.

For research purposes only, not for human use
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