GRIK5/GluK5 (extracellular) Blocking Peptide (#BLP-GC042) is the original antigen used for immunization during Anti-GRIK5 (GluK5) (extracellular) Antibody (#AGC-042) generation. The blocking peptide binds and ‘blocks’ Anti-GRIK5/GluK5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
- Expression of kainate receptor GluK5 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-GRIK5 (GluK5) (extracellular) Antibody (#AGC-042), (1:300), followed by goat-anti-rabbit-Alexa-488. GluK5 staining (green) appears in neurons (horizontal arrows) of the pyramidal layer (Pyr) and in apical dendrites (vertical arrows) of mouse hippocampus. Nuclei are stained with DAPI (blue).
- (C)RRPNFQALSGNER,corresponding to amino acid residues 430-442 of rat kainate receptor GluK5 (Accession Q63273).