Overview
Type: Synthetic peptide
Form: Lyophilized powder
GRIK5/GluK5 (extracellular) Blocking Peptide (#BLP-GC042) is the original antigen used for immunization during Anti-GRIK5 (GluK5) (extracellular) Antibody (#AGC-042) generation. The blocking peptide binds and ‘blocks’ Anti-GRIK5/GluK5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1,2. Anti-GRIK5 (GluK5) (extracellular) Antibody (#AGC-042), (1:400).
3,4. Anti-GRIK5 (GluK5) (extracellular) Antibody, preincubated with GRIK5/GluK5 (extracellular) Blocking Peptide (#BLP-GC042).
Expression of kainate receptor GluK5 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-GRIK5 (GluK5) (extracellular) Antibody (#AGC-042), (1:300), followed by goat-anti-rabbit-Alexa-488. GluK5 staining (green) appears in neurons (horizontal arrows) of the pyramidal layer (Pyr) and in apical dendrites (vertical arrows) of mouse hippocampus. Nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RRPNFQALSGNER,corresponding to amino acid residues 430-442 of rat kainate receptor GluK5 (Accession Q63273).
Accession (Uniprot) Number Q63273
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
