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Guinea Pig Anti-Alpha-Synuclein Antibody

α-Synuclein, Non-A4 component of amyloid precursor, Parkinson disease (autosomal dominant, Lewy body) 4, PARK1, PARK4, NACP, SNCA

Cat #: AGP-169
Alternative Name α-Synuclein, Non-A4 component of amyloid precursor, Parkinson disease (autosomal dominant, Lewy body) 4, PARK1, PARK4, NACP, SNCA
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)KDQMGKGEEGYPQE, corresponding to amino acid residues 97-110 of rat α-synuclein (Accession P37377). Intracellular, C-terminus.
Accession (Uniprot) Number P37377
Gene ID SNCA
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 11 out of 14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1-2. Guinea Pig Anti-Alpha-Synuclein Antibody (#AGP-169), (1:400).
    3-4. Guinea Pig Anti-Alpha-Synuclein Antibody, preincubated with Alpha-Synuclein Blocking Peptide (BLP-PZ035).
Immunohistochemistry
  • Expression of Alpha-Synuclein in rat hippocampus.
    Expression of Alpha-Synuclein in rat hippocampus.
    Expression of Alpha-Synuclein in rat hippocampus. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-Alpha-Synuclein Antibody (#AGP-169), (1:300), followed by goat anti-guinea pig-AlexaFluor-594. A. Alpha-Synuclein immunoreactivity (red) appears in the mossy fiber terminal region (MFT). B. Pre-incubation of the antibody with Alpha-Synuclein Blocking Peptide (BLP-PZ035), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer.


References
  1. Beyer, K. (2006) Acta. Neurophatol. 112, 237.
  2. Auluck, P.K. et al. (2010) Annu. Rev. Cell Dev. Biol. 26, 211.
Scientific background

α-Synuclein belongs to the Synuclein family of proteins containing three other members (β-synuclein, γ-synuclein and synoretnin). α-Synuclein is a highly conserved acidic protein that is concentrated mainly in presynaptic axon terminals.

The gene encoding α-synuclein is known as SNCA or PARK1. α-Synuclein, together with other members of its family, lacks typical secondary structure. In its primary structure, α-synuclein is characterized by seven imperfect 11-residue repeat sequences that are predicted to form five amphipatic helices on the amino terminal half. The acidic, glutamate rich carboxy-terminal region remains unstructured even in the presence of membranes. α-Synuclein helices 1-4 are predicted to associate with lipid vesicles while helix 5 would be responsible for protein-to-protein interaction. The protein interacts and with DAT, the dopamine transporter, and thus is involved in dopamine signaling. Under pathological conditions, α-synuclein can undergo conformational changes leading to protein aggregation and deposition.

α-Synuclein shares structural homology with the 14-3-3 family of chaperon proteins and its expression is upregulated during periods of accelerated neuronal activity and plasticity thus suggesting it might be functioning as a molecular chaperon capable of binding to other intercellular proteins.

α-Synuclein has been implicated in the pathophysiology of Parkinson’s disease (PD) and Lewy body dementia (DLB). Mutations A30P and A3T are associated with PD while mutation E46K has been linked to DLB. These mutated α-synucleins form fibrils more rapidly than the wild-type protein in aqueous solution1. α-Synucleins are the major components of Lewy bodies and Lewy neurites in both diseases2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 02/03/2021

Guinea Pig Anti-Alpha-Synuclein Antibody (#AGP-169) is a highly specific antibody directed against an epitope of rat α-Synuclein (SNCA). The antibody can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize α-Synuclein from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-Alpha-Synuclein Antibody (#APZ-035) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

For research purposes only, not for human use
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