Hemitoxin

K+ channel toxin α-KTx 6.15
A Potent Blocker of KV1.1, KV1.2 and KV1.3 K+ Channels
Cat #: STH-500
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Hemiscorpius lepturus (scorpion).
    Source Synthetic peptide
    MW: 3898 Da.
    Purity: >98% (HPLC)
    Effective concentration 1 nM - 1 μM.
    Sequence IKCTLSKDCYSPCKKETGCPRAKCINRNCKCYGCS.
    Modifications Disulfide bonds between Cys3-Cys24, Cys9-Cys29, Cys13-Cys19 and Cys31-Cys34.
    Molecular formula C159H259N49O49S8.
    Activity Hemitoxin reversibly inhibits rat voltage-gated K+ channels KV1.1 (KCNA1), KV1.2 (KCNA2) and KV1.3 (KCNA3)1.
    References - Activity
    1. Srairi-Abid, N. et al. (2008) FEBS J. 275, 4641.

    Storage before reconstitution Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Reconstitution Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage after reconstitution Up to one week at 4°C or three months at -20°C.
    Our bioassay
    Alomone Labs Hemitoxin inhibits KV1.2 channel currents heterologously expressed in Xenopus oocytes.
    Currents were elicited by application of a 100 msec voltage ramp from a holding potential of -80 mV to 60 mV, delivered every 10 seconds. A. Time course of channel activity (current amplitude at -20 mV), before and during application of 500 and 100 nM Hemitoxin (#STH-500) as indicated by the bars. B. Example of superimposed current traces before and during application of 500 and 100 nM Hemitoxin (taken from the experiment in A).
    References - Scientific Background
    1. Srairi-Abid, N. et al. (2008) FEBS J. 275, 4641.
    Scientific background Hemitoxin is a 35 amino acid peptidyl toxin isolated from the Hemiscorpius lepturus scorpion venom and belongs to the αKTx6 family1. It reversibly blocks KV1.1, KV1.2 and KV1.3 voltage-gated K+ channels with an IC50 of 13 nM, 16 nM and 2 nM, respectively1. It shares 80% sequence similarity with Maurotoxin, and has a unique pairing of its four disulfide bridges1.
    Target KV1.1, KV1.2, KV1.3 K+ channels
    Net Peptide Content: 100%
    Last update: 03/03/2019

    Hemitoxin (#STH-500) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use
    Citations
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