Every lot is tried & tested in a relevant biological assay.
BSA (0.1 mg/ml) should be added for more diluted solutions. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
- Alomone Labs Recombinant human LIF protein activates ERK1/2 MAPK and STAT3 in 3T3-L1 cells.Cells were serum starved for 2 h and stimulated with 100 ng/ml of Recombinant human LIF protein (#L-200) for 10 min. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-STAT3 and anti-phospho-ERK1/2.
- Brach, M.A. et al. (1990) Leukemia 4, 646.
- Estrov, Z. et al. (1992) Leuk. Lymphoma 8, 1.
- Gough, N.M. et al. (1989) Cancer Cells. 1, 77.
- Metcalf, D. (2003) Stem Cells 21, 5.
- Zurn, A.D. and Werren, F. (1994) Dev. Biol. 163, 309.
- Martinou, J.C. et al. (1992) Neuron 8, 737.
- Gadient, R.A. and Patterson, P.H. (1999) Stem Cells 17, 127.
- Patterson, P.H. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 7833.
- Gabay, C. et al. (1996) Clin. Exp. Immunol. 105, 260.
- Lass, A. et al. (2001) Fertil. Steril. 76, 1091.
- Robb, L. et al. (2002) J. Reprod. Immunol. 57, 129.
- Ledee Bataille, N. et al. (2002) Hum. Reprod. 17, 213.
- Vos, J.P. et al. (1996) Perspect. Dev. Neurobiol. 4, 39.
- Butzkueven, H. et al. (2002) Nat. Med. 8, 613.
- Horton, A.R. et al. (1996) Perspect. Dev. Neurobiol. 4, 35.
- Murphy, M. et al. (1997) Prog. Neurobiol. 52, 355.
- Rajan, P. et al. (1995) Neuroreport 6, 2240.
- Akita, S. et al. (1996) J. Clin. Endocrinol. Metab. 81, 4170.
Leukemia inhibitory factor (LIF) was identified by its ability to induce terminal differentiation in leukemic cells.1,2 LIF is a pleiotrophic factor with known actions in the immune system, the nervous system and the reproductive system.3,4
In the nervous system it acts on cultured sympathetic neurons to direct a change in neurotransmitter expression from a noradrenergic to a cholinergic phenotype and regulates the expression of neuropeptide transmitters in these cells.5,6
In the immune system LIF plays a key role in inflammation,7,8 with a pro-inflammatory role in rheumatoid arthritis,9 but also with proposed anti-inflammatory properties in lung inflammatory processes.
In the reproductive system, LIF appears to play an important role in implantation and in the establishment of pregnancy.10,12 LIF acts as a trophic factor for oligodendrocytes and promotes astrocytic survival and differentiation.13,14 It exhibits activity towards spinal motor neurons and stimulate the biosynthesis of acetylcholine.6
LIF also functions as a trophic factor for peripheral sensory neurons supporting their survival.15,16 LIF appears to be essential for injury-induced neuropeptide synthesis17 and can also stimulate the hypothalamic-pituitary-adrenal axis in response to stress and disease.18 LIF is used extensively in experimental biology because of its ability to induce embryonic stem cells to retain their totipotentiality.
Recombinant human LIF protein (#L-200) is a highly pure, recombinant, and biologically active protein.