Human proNGF Deluxe Research Pack

All You Need for proNGF Research
  • Lyophilized Powder
  • Antigen Incl.
  • Fluorescent Label
  • Bioassay Tested
  • QC Tested
  • Shipped at Room Temp.
  • Live Cell Imaging
  • Immuno-Colocalization
  • 100% Net Peptide
  • Sterile & Endotoxin Free
Cat #: ESD-652
Sizes: 11 Vials
Last update: 01/07/2018

Alomone Labs is pleased to offer the Human proNGF Deluxe Research Pack (#ESD-652). The Research Pack contains all you need for human proNGF research: Antibodies to proNGF and its receptor, and recombinant proteins all in one economical package!

For research purposes only, not for human use
Compounds
Product NameCat #Size
Anti-proNGF Antibody
ANT-005 1 x 0.2 ml
Guinea pig Anti-proNGF Antibody
AGP-031 1 x 0.2 ml
Anti-p75 NGF Receptor (extracellular) Antibody
ANT-007 1 x 0.2 ml
Anti-p75 NGF Receptor (extracellular)-ATTO-488 Antibody
ANT-007-AG 1 x 50 µl
Mouse NGF/proNGF Neutralizing Antibody
ALM-006 1 x 0.25 mg
human proNGF
N-280 1 x 25 µg
human proNGF (cleavage resistant)
N-285 1 x 10 µg
Note A guinea pig polyclonal antibody (#AGP-031) and mouse monoclonal antibody (#ALM-006) are included in this Research Pack. Please take into account when reacting with a secondary antibody.
Scientific Background
Scientific Background

Neurotrophins are synthesized as pro-forms that can be cleaved either intracellularly to release mature, secreted ligands, or extracellularly by various proteases such as plasmin, furin, PC1/3, PC7, and PACE 4.1,2,5 The immature precursor has a prodomain of 103 amino acids, which was thought to have a role in the folding and sorting of the mature NGF into the various secretion pathways. 

It was recently reported that proNGF, binds p75NTR receptor preferentially over TrkA, and this selective binding of proNGF to p75NTR  leads to apoptotic death of cells that express both TrkA and p75NTR. However, mature NGF binds and activates both receptors, with resulting promotion of cell survival due to the TrkA-mediated survival signal overriding p75NTR -mediated apoptotic signal.3,4 

Since pro- and mature neurotrophins seem to elicit opposite functional effects, by differential interactions with Trks and p75NTR receptors, extracellular cleavage represents a new way to control the synaptic functions of neurotrophins.

It was demonstrated that proNGF from injured spinal cord extracts, is active and induce apoptosis among oligodendrocytes, and apoptosis can be blocked by a proNGF-specific antibody.6  

Finally, proNGF was demonstrated as the predominant form in mouse, rat, and human brain tissue, thyroid, hippocampus, thus suggesting a role for proNGF in vivo.5,7,8

References
  1. Lessman, V. et al. (2003) Prog. Neurobiol. 69, 341.
  2. Bibel, M. and Barde, Y.A. et al. (2000) Genes Dev. 14, 2929.
  3. Lee, R. et al. (2001) Science 294, 1945.
  4. Ibanez, C.F. (2002) Trends Neurosci. 25, 284.
  5. Hasan, W. et al. (2003) J. Neurobiol. 57, 38.
  6. Beattie, M.S. et al. (2002) Neuron 36, 375.
  7. Fahnestock, M. et al. (2001) Mol. Cell. Neurosci. 18, 210.
  8. Lu, B. (2003) Neuron 39, 735.
Related Products