KCNQ1 Blocking Peptide (#BLP-PC022) is the original antigen used for immunization during Anti-KCNQ1 Antibody (#APC-022) generation. The blocking peptide binds and ‘blocks’ Anti-KCNQ1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat heart membranes:
- Mouse distal colon (1:50) (Jepps, T.A. et al. (2009) Am. J. Physiol. 297, G107.).
- Western blot analysis of rat heart membrane (lanes 1 and 3), rat spleen lysate (lanes 2 and 4), mouse heart membrane (lanes 5 and 6) and human Colo205 cell line lysate (lanes 7 and 8):
- Expression of KCNQ1 in mouse heartImmunohistochemical staining of mouse heart frozen section using Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), (1:200). A. KCNQ1 staining (red) appears in muscle fiber membranes (arrows). B. Nuclei are stained using DAPI counterstain (blue). C. Merged image of panels A and B.
- (C)TYEQLTVPRRGPDEGS, corresponding to amino acid residues 661-676 of human KCNQ1 (Accession P51787).