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Anti-KCNQ1 Antibody

KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1

Cat #: APC-022
Alternative Name KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)TYEQLTVPRRGPDEGS, corresponding to amino acid residues 661-676 of human KCNQ1 (Accession P51787). Intracellular, C-terminus.
Accession (Uniprot) Number P51787
Gene ID 3784
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - 14/16 amino acid residues identical.
RRID AB_2040099.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.75 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, ip, wb
May also work in: ifc*
Western blot
  • Western blot analysis of rat heart membranes:
    Western blot analysis of rat heart membranes:
    1. Anti-KCNQ1 Antibody (#APC-022), (1:200).   
    2. Anti-KCNQ1 Antibody, preincubated with KCNQ1 Blocking Peptide (#BLP-PC022).
Immunoprecipitation
  • Canine left ventricle (8 μg) (Ehrlich, J.R. et al. (2004) J. Biol. Chem. 279, 1233.).
Immunohistochemistry
  • Mouse distal colon (1:50) (Jepps, T.A. et al. (2009) Am. J. Physiol. 297, G107.).
Immunocytochemistry
  • COS cells (Wiener, R. et al. (2008) J. Biol. Chem. 283, 5815.).
References
  1. Wang, Q. et al. (1996) Nat. Genet. 12, 17.
  2. Grahammer, F. et al. (2001) Gastroenterology 120, 1363.
  3. Mall, M. et al. (1998) Am. J. Physiol. 275, G1274.
Scientific background

KCNQ1 (KV7.1) is part of a voltage-gated K+ family that includes 5 other members (KCNQ1-5).  

The channel has been intensively studied since it was found that together with MinK (Isk) it underlay the cardiac Iks current that controls the duration of the action potential of the human heart. Indeed mutations on either KV7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death. 

Although KV7.1 expression and function has been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues. There it has been implicated in physiological functions such as the regulation of acid secretion in the stomach and Cl- secretion into the colon.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Image & Title:

Anti-KCNQ1 Antibody Expression of KCNQ1 (KV7.1) in hiPSC-CMs.Western blot analysis of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) under different treatments using Anti-KCNQ1 Antibody (#APC-022). Protein levels of KCNQ1 remain the same.Adapted from Piccini, I. et al. (2017) Front. Physiol. 8, 705. with permission of Frontiers.

Last update: 13/10/2021

Anti-KCNQ1 Antibody (#APC-022) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitaion, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize KV7.1 channel from rat, human, and mouse samples.

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For research purposes only, not for human use

Applications

Citations

Citations
Published figures using this product
  • Expression of KCNQ1 (KV7.1) and KCNQ5 (KV7.5) in rat smooth muscle cells.
    Expression of KCNQ1 (KV7.1) and KCNQ5 (KV7.5) in rat smooth muscle cells.
    Immunocytochemical staining of rat smooth muscle cells from LCA and RCA using Anti-KCNQ1 Antibody (#APC-022) and Anti-KCNQ5 Antibody (#APC-155) shows that the expression of both channels is higher in LCA than in RCA.
    Adapted from Morales-Cano, D. et al. (2015) with permission of the European Society of Cardiology.
Western blot citations
  1. Guinea pig heart lysate.
    Zankov, D.P. et al. (2019) Heart Rhythm 16, 108.
  2. Rat heart lysate (1:2000).
    Masuda, K. et al. (2018) J. Physiol. Sci. 68, 759.
  3. CHO and HEK 293 transfected cells.
    Meisel, E. et al. (2018) Channels 12, 89.
  4. Human detrusor lysate.
    Bientinesi, R. et al. (2017) Naunyn-Schmiedebergs Arch. Pharmacol. 390, 127.
  5. HEK 293 transfected cells.
    Moreno, C. et al. (2017) J. Mol. Cell. Cardiol. 110, 61.
  6. Human heart tissue sample lysate (1:200).
    Opthof, T. et al. (2017) Heart Rhythm 14, 265.
  7. Human induced pluripotent stem cell-derived cardiomyocytes.
    Piccini, I. et al. (2017) Front. Physiol. 8, 705.
  8. HEK 293 transfected cells.
    Inanobe, A. et al. (2015) Cell. Physiol. Biochem. 36, 1847.
  9. Mouse transfected MDCK cells (1:1000).
    Andersen, M.N. et al. (2015) Am. J. Physiol. 309, C693.
  10. Mouse mMCD3 cell lysate (1:300).
    Slaats, G.G. et al. (2015) J. Cell. Sci. 128, 4550.
  11. MDCK cells stably expressing human KV7.1 (1:1000).
    Andersen, M.N. et al. (2013) J. Biol. Chem. 288, 36841.
  12. Xenopus oocyte membrane lysates (1:500).
    Lundby, A. et al. (2013) Sci. Signal. 6, rs11.
Immunoprecipitation citations
  1. HEK 293 transfected cells.
    Inanobe, A. et al. (2015) Cell. Physiol. Biochem. 36, 1847.
  2. Mouse transfected MDCK cells.
    Andersen, M.N. et al. (2015) Am. J. Physiol. 309, C693.
  3. Canine left ventricle (8 μg).
    Ehrlich, J.R. et al. (2004) J. Biol. Chem. 279, 1233.
Immunohistochemistry citations
  1. Rat rectal colon sections.
    Inagaki, A. et al. (2019) Pflugers Arch. 471, 313.
  2. Mouse cochlea sections (1:100).
    Cazals, Y. et al. (2015) Nat. Commun. 6, 8780.
  3. Rat kidney sections (1:200).
    Slaats, G.G. et al. (2015) J. Cell. Sci. 128, 4550.
  4. Guinea pig detrusor (1:200).
    Anderson, U.A. et al. (2013) Br. J. Pharmacol. 169, 1290.
  5. Mouse distal colon (1:50).
    Jepps, T.A. et al. (2009) Am. J. Physiol. 297, G107.
Immunocytochemistry citations
  1. Guinea pig myocytes.
    Zankov, D.P. et al. (2019) Heart Rhythm 16, 108.
  2. Mouse mMCD3 cells (1:200).
    Slaats, G.G. et al. (2015) J. Cell. Sci. 128, 4550.
  3. Rat smooth muscle cells (1:100).
    Morales-Cano, D. et al. (2015) Cardiovasc. Res. 106, 98.
  4. COS cells.
    Wiener, R. et al. (2008) J. Biol. Chem. 283, 5815.
More product citations
  1. Lundby, A. et al. (2013) Sci. Signal. 6, rs11.
  2. Askar, S.F. et al. (2013) Cardiovasc. Res. 97, 171.
  3. Organ-Darling, L.E. et al. (2013) Am. J. Physiol. 304, H589.
  4. Alzamora, R. et al. (2010) Am. J. Physiol. 299, F1308.
  5. Roura-Ferrer, M. et al. (2010) J. Cell. Physiol. 225, 692.
  6. Roura-Ferrer, M. et al. (2008) Biochem. Biophys. Res. Com. 369, 1094.
  7. Xiao, L. et al (2008) Circulation 118, 983.
  8. Yeung, S.Y. et al. (2008) Br. J. Pharmacol. 155, 62.
  9. Yeung, S.Y. et al. (2007) Br. J. Pharmacol. 151, 758.

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