Anti-KCNQ1 Antibody

KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1
    Cat #: APC-022
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)TYEQLTVPRRGPDEGS, corresponding to amino acid residues 661-676 of human KCNQ1 (Accession P51787). Intracellular, C-terminus.
    Accession (Uniprot) Number P51787
    Gene ID 3784
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 14/16 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 2.5 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, ip, wb
    May also work in: ifc
    Western blot
    Western blot analysis of rat heart membranes:
    1. Anti-KCNQ1 Antibody (#APC-022), (1:200).   
    2. Anti-KCNQ1 Antibody, preincubated with the control peptide antigen.
    Immunoprecipitation
    Canine left ventricle (8 μg) (Ehrlich, J.R. et al. (2004) J. Biol. Chem279, 1233.).
    Immunohistochemistry
    Mouse distal colon (1:50) (Jepps, T.A. et al. (2009) Am. J. Physiol. 297, G107.).
    Immunocytochemistry
    COS cells (Wiener, R. et al. (2008) J. Biol. Chem. 283, 5815.).
    References
    1. Wang, Q. et al. (1996) Nat. Genet. 12, 17.
    2. Grahammer, F. et al. (2001) Gastroenterology 120, 1363.
    3. Mall, M. et al. (1998) Am. J. Physiol. 275, G1274.
    Scientific background

    KCNQ1 (KV7.1) is part of a voltage-gated K+ family that includes 5 other members (KCNQ1-5).  

    The channel has been intensively studied since it was found that together with MinK (Isk) it underlay the cardiac Iks current that controls the duration of the action potential of the human heart. Indeed mutations on either KV7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death. 

    Although KV7.1 expression and function has been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues. There it has been implicated in physiological functions such as the regulation of acid secretion in the stomach and Cl- secretion into the colon.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-KCNQ1 Antibody Expression of KCNQ1 (KV7.1) in hiPSC-CMs.Western blot analysis of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) under different treatments using Anti-KCNQ1 Antibody (#APC-022). Protein levels of KCNQ1 remain the same.Adapted from Piccini, I. et al. (2017) Front. Physiol. 8, 705. with permission of Frontiers.

    Last update: 31/12/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of human KCNQ1 (KV7.1). Anti-KCNQ1 Antibody (#APC-022) can be used in western blot, immunoprecipitaion, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize KCNQ1 from rat, human, and mouse samples.

    For research purposes only, not for human use
    Citations
    Published figures using this product
    Expression of KCNQ1 (KV7.1) and KCNQ5 (KV7.5) in rat smooth muscle cells.
    Immunocytochemical staining of rat smooth muscle cells from LCA and RCA using Anti-KCNQ1 Antibody (#APC-022) and Anti-KCNQ5 Antibody (#APC-155) shows that the expression of both channels is higher in LCA than in RCA.
    Adapted from Morales-Cano, D. et al. (2015) with permission of the European Society of Cardiology.
    Western blot citations
    1. Human induced pluripotent stem cell-derived cardiomyocytes.
      Piccini, I. et al. (2017) Front. Physiol. 8, 705.
    2. HEK 293 transfected cells.
      Inanobe, A. et al. (2015) Cell. Physiol. Biochem. 36, 1847.
    3. Mouse transfected MDCK cells (1:1000).
      Andersen, M.N. et al. (2015) Am. J. Physiol. 309, C693.
    4. Mouse mMCD3 cell lysate (1:300).
      Slaats, G.G. et al. (2015) J. Cell. Sci. 128, 4550.
    5. MDCK cells stably expressing human KV7.1 (1:1000).
      Andersen, M.N. et al. (2013) J. Biol. Chem. 288, 36841.
    6. Xenopus oocyte membrane lysates (1:500).
      Lundby, A. et al. (2013) Sci. Signal. 6, rs11.
    Immunoprecipitation citations
    1. HEK 293 transfected cells.
      Inanobe, A. et al. (2015) Cell. Physiol. Biochem. 36, 1847.
    2. Mouse transfected MDCK cells.
      Andersen, M.N. et al. (2015) Am. J. Physiol. 309, C693.
    3. Canine left ventricle (8 μg).
      Ehrlich, J.R. et al. (2004) J. Biol. Chem279, 1233.
    Immunohistochemistry citations
    1. Mouse cochlea sections (1:100).
      Cazals, Y. et al. (2015) Nat. Commun. 6, 8780.
    2. Rat kidney sections (1:200).
      Slaats, G.G. et al. (2015) J. Cell. Sci. 128, 4550.
    3. Guinea pig detrusor (1:200).
      Anderson, U.A. et al. (2013) Br. J. Pharmacol. 169, 1290.
    4. Mouse distal colon (1:50).
      Jepps, T.A. et al. (2009) Am. J. Physiol. 297, G107.
    Immunocytochemistry citations
    1. Mouse mMCD3 cells (1:200).
      Slaats, G.G. et al. (2015) J. Cell. Sci. 128, 4550.
    2. Rat smooth muscle cells (1:100).
      Morales-Cano, D. et al. (2015) Cardiovasc. Res. 106, 98.
    3. COS cells.
      Wiener, R. et al. (2008) J. Biol. Chem. 283, 5815.
    More product citations
    1. Lundby, A. et al. (2013) Sci. Signal. 6, rs11.
    2. Askar, S.F. et al. (2013) Cardiovasc. Res. 97, 171.
    3. Organ-Darling, L.E. et al. (2013) Am. J. Physiol. 304, H589.
    4. Alzamora, R. et al. (2010) Am. J. Physiol. 299, F1308.
    5. Roura-Ferrer, M. et al. (2010) J. Cell. Physiol. 225, 692.
    6. Roura-Ferrer, M. et al. (2008) Biochem. Biophys. Res. Com. 369, 1094.
    7. Xiao, L. et al (2008) Circulation 118, 983.
    8. Yeung, S.Y. et al. (2008) Br. J. Pharmacol. 155, 62.
    9. Yeung, S.Y. et al. (2007) Br. J. Pharmacol. 151, 758.
    Related Products
      • Products for control experiments
        1. Anti-KCNQ1 (extracellular) Antibody (#APC-168). This antibody recognizes a different epitope and thus can also be used as a control for detecting KCNQ1.
      • Antibodies for live cell imaging experiments
        1. Anti-KCNQ1 (extracellular) Antibody (#APC-168). This antibody recognizes an extracellular epitope and can be used to detect KCNQ1 in live cells.