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Guinea pig Anti-KCNQ1 Antibody

KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KVLQT1

Cat #: APC-022-GP (formerly AGP-050)
Alternative Name KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KVLQT1
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea Pig
Reactivity: h, m, r
Immunogen
  • Peptide (C)TYEQLTVPRRGPDEGS, corresponding to amino acid residues 661-676 of human KCNQ1 (Accession P51787). Intracellular, C-terminus.
Accession (Uniprot) Number P51787
Gene ID 3784
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - 14/16 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat heart membrane (lanes 1 and 3), rat spleen lysate (lanes 2 and 4), mouse heart membrane (lanes 5 and 6) and human Colo205 cell line lysate (lanes 7 and 8):
    Western blot analysis of rat heart membrane (lanes 1 and 3), rat spleen lysate (lanes 2 and 4), mouse heart membrane (lanes 5 and 6) and human Colo205 cell line lysate (lanes 7 and 8):
    1,2,5,7. Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), (1:200).
    3,4,6,8. Guinea pig Anti-KCNQ1 Antibody, preincubated with KCNQ1 Blocking Peptide (#BLP-PC022).
Immunohistochemistry
  • Expression of KCNQ1 in mouse heart
    Expression of KCNQ1 in mouse heart
    Immunohistochemical staining of mouse heart frozen section using Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), (1:200). A. KCNQ1 staining (red) appears in muscle fiber membranes (arrows). B. Nuclei are stained using DAPI counterstain (blue). C. Merged image of panels A and B.
References
  1. Wang, Q. et al. (1996) Nat. Genet. 12, 17.
  2. Grahammer, F. et al. (2001) Gastroenterology 120, 1363.
  3. Mall, M. et al. (1998) Am. J. Physiol. 275, G1274.
Scientific background

KCNQ1 (KV7.1) is part of a voltage-gated K+ family that includes 5 other members (KCNQ1-5).

The channel has been intensively studied since it was found that together with MinK (Isk) it is responsible for the cardiac Iks current that controls the duration of the action potential of the human heart. Indeed, mutations in either KV7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death1-3.

Although KV7.1 expression and function have been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues where it is involved in physiological functions such as the regulation of acid secretion in the stomach and Cl- secretion into the colon1-3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 08/01/2023

Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), raised in guinea pigs, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV7.1 channel from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-KCNQ1 Antibody (#APC-022) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

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For research purposes only, not for human use

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