- Peptide (C)TYEQLTVPRRGPDEGS, corresponding to amino acid residues 661-676 of human KCNQ1 (Accession P51787). Intracellular, C-terminus.
- Western blot analysis of rat heart membrane (lanes 1 and 3), rat spleen lysate (lanes 2 and 4), mouse heart membrane (lanes 5 and 6) and human Colo205 cell line lysate (lanes 7 and 8):1,2,5,7. Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), (1:200).
3,4,6,8. Guinea pig Anti-KCNQ1 Antibody, preincubated with KCNQ1 Blocking Peptide (#BLP-PC022).
- Expression of KCNQ1 in mouse heartImmunohistochemical staining of mouse heart frozen section using Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), (1:200). A. KCNQ1 staining (red) appears in muscle fiber membranes (arrows). B. Nuclei are stained using DAPI counterstain (blue). C. Merged image of panels A and B.
KCNQ1 (KV7.1) is part of a voltage-gated K+ family that includes 5 other members (KCNQ1-5).
The channel has been intensively studied since it was found that together with MinK (Isk) it is responsible for the cardiac Iks current that controls the duration of the action potential of the human heart. Indeed, mutations in either KV7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death1-3.
Although KV7.1 expression and function have been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues where it is involved in physiological functions such as the regulation of acid secretion in the stomach and Cl- secretion into the colon1-3.
Species reactivity key:
Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), raised in guinea pigs, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV7.1 channel from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-KCNQ1 Antibody (#APC-022) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.
- Anti-KCNQ1 (extracellular) Antibody (#APC-168). This antibody recognizes an extracellular epitope and can be used to detect KCNQ1 in live cells.