Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNT1/Slack Blocking Peptide (#BLP-PC124) is the original antigen used for immunization during Anti-KCNT1 (Slack) Antibody (#APC-124) generation. The blocking peptide binds and ‘blocks’ Anti-KCNT1/Slack primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane:1. Anti-KCNT1 (Slack) Antibody (#APC-124), (1:200).
2. Anti-KCNT1 (Slack) Antibody, preincubated with KCNT1/Slack Blocking Peptide (#BLP-PC124).
Western blot analysis of mouse brain membrane:1. Anti-KCNT1 (Slack) Antibody (#APC-124), (1:200).
2. Anti-KCNT1 (Slack) Antibody, preincubated with KCNT1/Slack Blocking Peptide (#BLP-PC124).
Expression of KCNT1 (Slack) in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNT1 (Slack) Antibody (#APC-124), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. KCNT1 (Slack) immunoreactivity (green) appears in the molecular layer (MOL) and Purkinje cells (arrows). B. Pre-incubation of the antibody with KCNT1/Slack Blocking Peptide (#BLP-PC124), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KQEEKQNRRGLAG, corresponding to amino acid residues 619-631 of rat Slack (Accession Q9Z258).
Accession (Uniprot) Number Q9Z258
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
