Kv1.4 (extracellular) Blocking Peptide (#BLP-PC167) is the original antigen used for immunization during Anti-KV1.4 (extracellular) Antibody (#APC-167) generation. The blocking peptide binds and ‘blocks’ Anti-Kv1.4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate:
- Western blot analysis of mouse brain lysate:
- Expression of KV1.4 in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:400), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. KV1.4 staining (red) appears in several neuronal-outlined cells (arrows). Nuclei were stained with DAPI (blue).
- (C)GHSRLLNDTSAPHLE, corresponding to amino acid residues 348 - 362 of rat KV1.4 (Accession P15385).