Anti-KV1.4 (extracellular) Antibody

Potassium voltage-gated channel subfamily A member 4, KCNA4
    Cat #: APC-167
    Alternative Name Potassium voltage-gated channel subfamily A member 4, KCNA4
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
    • Peptide (C)GHSRLLNDTSAPHLE, corresponding to amino acid residues 348 - 362 of rat KV1.4 (Accession P15385). 1st extracellular loop.
    • Anti-KV1.4 (extracellular) Antibody
    Accession (Uniprot) Number P15385
    Gene ID 25469
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human – 13/15 amino acid residues identical.
    RRID AB_2756751.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*
    Western blot
    • Anti-KV1.4 (extracellular) Antibody
      Western blot analysis of rat brain lysate:
      1. Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:400).
      2. Anti-KV1.4 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-KV1.4 (extracellular) Antibody
      Western blot analysis of mouse brain lysate:
      1. Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:200).
      2. Anti-KV1.4 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-KV1.4 (extracellular) Antibody
      Expression of KV1.4 in rat parietal cortex
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:400), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. KV1.4 staining (red) appears in several neuronal-outlined cells (arrows). Nuclei were stained with DAPI (blue).
    Live cell imaging / Immunocytochemistry
    • Anti-KV1.4 (extracellular) Antibody
      Expression of KV1.4 in live intact rat PC12 cells
      Cell surface detection of KV1.4 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:25), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
    1. Stuhmer, W. et al. (1989) EMBO J. 8, 3235.
    2. Gutman, G.A. et al. (2005) Pharmacol. Rev. 57, 473.
    3. Bogin, O. (2006) Modulator 21, 28.
    Scientific background

    KV1.4 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.4 was first cloned from rat brain.1 Eight Shaker-related genes exist in mammals constituting the KV1 subfamily of the large KV channel family of genes.2

    A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition, several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.

    The structure of KV1.4 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2

    The channel is expressed in neurons and cardiac and skeletal muscle tissue as well as in the pancreas.2 The functional channel is considered transient (A-type) current and shows pronounced inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.

    KV1.4 channels are sensitive to high doses of TEA (>100 mM) and low doses of 4-AP (0.013 mM), the “classical” non-selective potassium channel blockers.

    Most venomous peptide toxins that affect other KV channels do not inhibit KV1.4. However, the sea anemone toxin Stichodactyla Toxin (ShK), which is more potent towards KV1.1 and KV1.3, is still a potent inhibitor of KV1.4 channels.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV1.4. Anti-KV1.4 (extracellular) Antibody (#APC-167) can be used in western blot, immunohistochemistry and live cell imaging applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize KV1.4 from human, rat and mouse samples.

    For research purposes only, not for human use
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