LPAR1/EDG2 (extracellular) Blocking Peptide (#BLP-LR031) is the original antigen used for immunization during Anti-LPAR1 (EDG2) (extracellular) Antibody (#ALR-031) generation. The blocking peptide binds and ‘blocks’ Anti-LPAR1/EDG2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membrane (lanes 1 and 3), mouse brain membrane (lanes 2 and 4) and human brain glioblastoma U87 MG cell line (lanes 5 and 6) lysate:
- Expression of LPAR1 in rat corpus callosum (CC).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-LPAR1 (EDG2) (extracellular) Antibody (#ALR-031), (1:1000), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. LPAR1 immunoreactivity (red) appears in CC cells with oligodendrocyte morphology (arrows). B. Pre-incubation of the antibody with LPAR1 (EDG2) (extracellular) Blocking Peptide (#BLP-LR031), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)RSGKYLATEWNTVSK, corresponding to amino acid residues 36-50 of rat LPA1 receptor (Accession P6179).