LRRK2 Blocking Peptide (#BLP-NR102) is the original antigen used for immunization during Anti-LRRK2 Antibody (#ANR-102) generation. The blocking peptide binds and ‘blocks’ Anti-LRRK2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- Expression of LRRK2 in rat striatum and substantia nigraImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-LRRK2 Antibody (#ANR-102), (1:400), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. A. LRKK2 staining (green) of striatum sections is detected in the cytoplasm of several striatal cells (arrows). B. Staining in the substantia nigra pars compacta shows LRKK2 staining (green) in nuclei and cytoplasm of several cells (arrows). Nuclei were stained with DAPI (blue).
- CKALHVLFERVSEE, corresponding to amino acid residues 183-196 of mouse LRRK2 (Accession Q5S006).