Nav1.7/SCN9A Blocking Peptide (#BLP-SC008) is the original antigen used for immunization during Anti-NaV1.7 (SCN9A) Antibody (#ASC-008) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.7/SCN9A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of DRG lysates:
- Western blot analysis of ND7/23 cell lysate (lanes 1,3) and rat brain membranes (lanes 2,4):
- Expression of NaV1.7 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-NaV1.7 (SCN9A) Antibody (#ASC-008 ). A. NaV1.7 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of NaV1.7 and Parvalbumin demonstrates colocalization (arrows).
- Western blot analysis of rat DRG lysates:
- (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562).