Overview
Nav1.7/SCN9A Blocking Peptide (#BLP-SC008) is the original antigen used for immunization during Anti-NaV1.7 (SCN9A) Antibody (#ASC-008) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.7/SCN9A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Application key:
Applications
- Western blot analysis of DRG lysates:1. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).
2. Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008). - Western blot analysis of ND7/23 cell lysate (lanes 1,3) and rat brain membranes (lanes 2,4):1,2. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).
3,4. Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008). - Expression of NaV1.7 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-NaV1.7 (SCN9A) Antibody (#ASC-008 ). A. NaV1.7 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of NaV1.7 and Parvalbumin demonstrates colocalization (arrows).
- Human cervical tissue (1:25) (Hernandez-Plata, E. et al. (2012) Int. J. Cancer 130, 2013.).
Mouse dorsal root ganglia (DRGs) (1:1000) (Amaya, F. et al. (2006) J. Neurosci. 26, 12852.). - Western blot analysis of rat DRG lysates:
1. Guinea pig Anti-NaV1.7 (SCN9A) Antibody (#ASC-008-GP), (1:400).
2. Guinea pig Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Note: addition of 0.5% Tween-20 to the primary antibody solution may strengthen the signal and lower background.
Properties
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