Overview
A Blocking peptide for Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody (#ASC-008-FR) and Guinea pig Anti-NaV1.7 (SCN9A) Antibody (#AGP-057). Intended to be used as a negative control.
Applications
Applications
Western blot analysis of DRG lysates:1. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).
2. Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Western blot analysis of ND7/23 cell lysate (lanes 1,3) and rat brain membranes (lanes 2,4):1,2. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).
3,4. Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Expression of NaV1.7 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-NaV1.7 (SCN9A) Antibody (#ASC-008 ). A. NaV1.7 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of NaV1.7 and Parvalbumin demonstrates colocalization (arrows).- Human cervical tissue (1:25) (Hernandez-Plata, E. et al. (2012) Int. J. Cancer 130, 2013.).
Mouse dorsal root ganglia (DRGs) (1:1000) (Amaya, F. et al. (2006) J. Neurosci. 26, 12852.). 
Expression of NaV1.7 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody (#ASC-008-FR). A. NaV1.7 staining (purple) appears in DRG neurons. B. Nuclear staining using DAPI as the counterstain. C. Merge images of A and B.
Western blot analysis of rat DRG lysates:1. Guinea pig Anti-NaV1.7 (SCN9A) Antibody (#AGP-057), (1:400).
2. Guinea pig Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Following a broad screen of secondary antibodies, the following was used for this application:
Western blot analysis:
#106-035-006 (Jackson ImmunoResearch).
Note: addition of 0.5% Tween-20 to the primary antibody solution may strengthen the signal and lower background.
Properties
Properties
- (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562).
Need Help With This Product?
Our reagent specialists are here to help you find the best product for your application. Please call or email us and we will be happy to help you find the right product for the job.
Call us at
+972 2587 2202 or Email Us
