Name: Nav1.7/SCN9A Blocking Peptide
Brand: Alomone Labs
SKU: BLP-SC008

Overview

Cat #: BLP-SC008

Type: Synthetic peptide

Form: Lyophilized powder

Nav1.7/SCN9A Blocking Peptide (#BLP-SC008) is the original antigen used for immunization during Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.7/SCN9A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Applications: wb, ihc

For research purposes only, not for human use

Applications

Demonstration of Pre-adsorption control

Western blot analysis of DRG lysates:
1. Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008), (1:200).
2. Anti-Na_V1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Western blot analysis of ND7/23 cell lysate (lanes 1,3) and rat brain membranes (lanes 2,4):
1,2. Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008), (1:200).
3,4. Anti-Na_V1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Expression of Na_V1.7 in rat DRG
Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008 ). A. Na_V1.7 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of Na_V1.7 and Parvalbumin demonstrates colocalization (arrows).
Human cervical tissue (1:25) (Hernandez-Plata, E. et al. (2012) Int. J. Cancer 130, 2013.).

Mouse dorsal root ganglia (DRGs) (1:1000) (Amaya, F. et al. (2006) J. Neurosci. 26, 12852.).
Western blot analysis of rat DRG lysates:
1. Guinea pig Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008-GP), (1:400).
2. Guinea pig Anti-Na_V1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).

Note: addition of 0.5% Tween-20 to the primary antibody solution may strengthen the signal and lower background.

Properties

Sequence

(C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562).

Accession (Uniprot) Number O08562

Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.

Purity >70%

Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.

Reconstitution 100 µl double distilled water (DDW).

Storage After Reconstitution -20°C.

Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.

Standard Quality Control Of Each Lot Western blot analysis.

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Shipping and Ordering information

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Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Na_V1.7 from rat, human, and mouse samples.

Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008)
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Na_V1.7 channel. Guinea pig Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008-GP) raised in guinea pigs can be used in western blot analysis. It has been designed to recognize Na_V1.7 from rat, human and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Guinea pig Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008-GP (formerly AGP-057))

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Order Summary

Nav1.7/SCN9A Blocking Peptide

Overview

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Applications

Properties

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