Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nav1.7/SCN9A Blocking Peptide (#BLP-SC008) is the original antigen used for immunization during Anti-NaV1.7 (SCN9A) Antibody (#ASC-008) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.7/SCN9A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of DRG lysates:1. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).
2. Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Western blot analysis of ND7/23 cell lysate (lanes 1,3) and rat brain membranes (lanes 2,4):1,2. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).
3,4. Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Expression of NaV1.7 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-NaV1.7 (SCN9A) Antibody (#ASC-008 ). A. NaV1.7 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of NaV1.7 and Parvalbumin demonstrates colocalization (arrows).- Human cervical tissue (1:25) (Hernandez-Plata, E. et al. (2012) Int. J. Cancer 130, 2013.).
Mouse dorsal root ganglia (DRGs) (1:1000) (Amaya, F. et al. (2006) J. Neurosci. 26, 12852.). 
Western blot analysis of rat DRG lysates:1. Guinea pig Anti-NaV1.7 (SCN9A) Antibody (#ASC-008-GP), (1:400).
2. Guinea pig Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Note: addition of 0.5% Tween-20 to the primary antibody solution may strengthen the signal and lower background.
Properties
Sequence
- (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562).
Accession (Uniprot) Number O08562
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
