Name: Nav1.7/SCN9A Blocking Peptide
Brand: Alomone Labs
SKU: BLP-SC008
Price: 245.00 USD
Availability: InStock

Overview

Cat #: BLP-SC008

Type: Synthetic peptide

Form: Lyophilized powder

A Blocking peptide for Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008), Anti-Na_V1.7 (SCN9A)-ATTO Fluor-633 Antibody (#ASC-008-FR) and Guinea pig Anti-Na_V1.7 (SCN9A) Antibody (#AGP-057). Intended to be used as a negative control.

Applications: bl

For research purposes only, not for human use

Citations

Applications

Blocking

Western blot analysis of DRG lysates:
1. Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008), (1:200).
2. Anti-Na_V1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Western blot analysis of ND7/23 cell lysate (lanes 1,3) and rat brain membranes (lanes 2,4):
1,2. Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008), (1:200).
3,4. Anti-Na_V1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Expression of Na_V1.7 in rat DRG
Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-Na_V1.7 (SCN9A) Antibody (#ASC-008 ). A. Na_V1.7 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of Na_V1.7 and Parvalbumin demonstrates colocalization (arrows).
Human cervical tissue (1:25) (Hernandez-Plata, E. et al. (2012) Int. J. Cancer 130, 2013.).

Mouse dorsal root ganglia (DRGs) (1:1000) (Amaya, F. et al. (2006) J. Neurosci. 26, 12852.).
Expression of Na_V1.7 in rat DRG
Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-Na_V1.7 (SCN9A)-ATTO Fluor-633 Antibody (#ASC-008-FR). A. Na_V1.7 staining (purple) appears in DRG neurons. B. Nuclear staining using DAPI as the counterstain. C. Merge images of A and B.
Western blot analysis of rat DRG lysates:
1. Guinea pig Anti-Na_V1.7 (SCN9A) Antibody (#AGP-057), (1:400).
2. Guinea pig Anti-Na_V1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).

Following a broad screen of secondary antibodies, the following was used for this application:
Western blot analysis:
#106-035-006 (Jackson ImmunoResearch).
Note: addition of 0.5% Tween-20 to the primary antibody solution may strengthen the signal and lower background.

Properties

Sequence

(C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562).

Accession (Uniprot) Number O08562

Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.

Purity >70%

Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.

Reconstitution 100 µl double distilled water (DDW).

Storage After Reconstitution -20°C.

Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.

Standard Quality Control Of Each Lot Western blot analysis.

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Order Summary

Nav1.7/SCN9A Blocking Peptide

Overview

Application key:

Citations

Applications

Applications

Properties

Properties

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