Anti-NaV1.7 (SCN9A) Antibody

Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
    Cat #: ASC-008
    Alternative Name Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
        Anti-NaV1.7 (SCN9A) Antibody
    Accession (Uniprot) Number O08562
    Gene ID 78956
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rabbit - 14/15 amino acid residues identical; human, mouse - 13/15 amino acid residues identical.
    RRID AB_2040198.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
      • Anti-NaV1.7 (SCN9A) Antibody
        Western blot analysis of DRG lysates:
        1. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).
        2. Anti-NaV1.7 (SCN9A) Antibody, preincubated with the control peptide antigen.
        Anti-NaV1.7 (SCN9A) Antibody
        Western blot analysis of ND7/23 cell lysate (lanes 1,3) and rat brain membranes (lanes 2,4):
        1,2. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).
        3,4. Anti-NaV1.7 (SCN9A) Antibody, preincubated with the control peptide antigen.
      • Anti-NaV1.7 (SCN9A) Antibody
        Expression of NaV1.7 in rat DRG
        Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-NaV1.7 (SCN9A) Antibody (#ASC-008 ). A. NaV1.7 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of NaV1.7 and Parvalbumin demonstrates colocalization (arrows).
        Human cervical tissue (1:25) (Hernandez-Plata, E. et al. (2012) Int. J. Cancer 130, 2013.).

        Mouse dorsal root ganglia (DRGs) (1:1000) (Amaya, F. et al. (2006) J. Neurosci. 26, 12852.).
      • Anti-NaV1.7 (SCN9A) Antibody
        Expression of NaV1.7 in rat DRG primary culture
        Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture. Cells were stained using Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody.
        Human cervical cancer cell line (1:25) (Hernandez-Plata, E. et al. (2012) Int. J. Cancer 130, 2013.).
    References
      • Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3.

        Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,8.

        NaV1.7 is predominantly expressed in dorsal root ganglions (DRG) of the peripheral nervous system. Dominant gain of function mutations in the NaV1.7 gene are associated with erythermalgia (a rare autosomal disease characterized by sporadic burning pain accompanied by redness and heat in the extremities).9-11 Loss of function mutations in NaV1.7 channels leads to complete ablation of pain perception in humans.11 These recent findings highlight the role of this NaV isoform and the subset of DRG neurons that express this channel in physiological pain sensation.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-Nav1.7 (SCN9A) Antibody
    Expression of NaV1.7 in mouse DRGs.Immunohistochemical staining of mouse DRG sections using Anti-NaV1.7 (SCN9A) Antibody (#ASC-008). NaV1.7 staining (red) is detected in DRGs. Fast Blue (FB) is used to label neurons.Adapted from Feng, B. et al. (2015) J. Neurophysiol. 113, 2618. with permission of The American Physiological Society.

    Last update: 10/11/2019

    Anti-NaV1.7 (SCN9A) Antibody (#ASC-008) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.7 from rat, human, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Rat DRG lysates (1:100).
        Sun, J. et al. (2018) Mol. Pain 14, 1.
      • Rat trigeminal ganglion lysate.
        Yang, K.Y. et al. (2016) J. Dent. Res. 95, 1183.
      • Rat brain neurolemma lysate.
        Murenzi, E. et al. (2016) Neurotoxicology 60, 260.
      • Rat DRG lysates (1:200).
        Yan, J. et al. (2015) Eur. Spine J. 25, 177.
      • Mouse DRG, PN and colon lysates.
        Feng, B. et al. (2015) J. Neurophysiol. 113, 2618.
      • Rat DRG lysate (1:200).
        Cheng, K.I. et al. (2014) Eur. J. Pain 18, 162.
      • Rat DRG lysates (1:100).
        Zhang, J.L. et al. (2013) Brain Res. 1493, 13.
      • Rat colon lysate.
        Wang, Y. et al. (2012) PLoS ONE 7, e53165.
      • Rat DRG lysates (1:100).
        Sun, W. et al. (2012) Brain 135, 359.
      • Rat DRG sections (1:500).
        Li, Y. et al. (2018) J. Neurosci. 38, 1124.
      • Rat DRG sections (1:200).
        Sun, J. et al. (2018) Mol. Pain 14, 1.
      • Mouse muscle spindle sections.
        Carrasco, D.I. et al. (2017) J. Neurophysiol. 117, 1690.
      • Rat trigeminal ganglion sections.
        Yang, K.Y. et al. (2016) J. Dent. Res. 95, 1183.
      • Rat lumbar spinal cord sections.
        Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
      • Rat DRG sections (1:100).
        Liu, Z. et al. (2016) Mol. Pain 12, 1.
      • Mouse L6 DRG and distal colorectum sections (1:1000).
        Feng, B. et al. (2015) J. Neurophysiol. 113, 2618.
      • Rat DRG sections (1:1000).
        Mukai, M. et al. (2014) Eur. Spine J. 23, 463.
      • Rat DRG sections (1:200).
        Zhang, J.L. et al. (2013) Brain Res. 1493, 13.
      • Rat DRG sections (1:200).
        Sun, W. et al. (2012) Brain 135, 359.
      • Human cervical tissue (1:25).
        Hernandez-Plata, E. et al. (2012) Int. J. Cancer 130, 2013.
      • Mouse dorsal root ganglia (DRGs) (1:1000).
        Amaya, F. et al. (2006) J. Neurosci. 26, 12852.
      • Human mammary epithelial cells (1:200).
        Warnier, M. et al. (2018) Aging Cell 17, e12736.
      • Mouse dissociated DRGs (1:1000).
        Yamane, M. et al. (2017) J. Cell. Sci. 130, 1393.
      • Human cervical cancer cell line (1:25).
        Hernandez-Plata, E. et al. (2012) Int. J. Cancer 130, 2013.
      • Qu, R. et al. (2013) Am. J. Physiol. 304, G763.
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